Instructions for Instruction

(version number: V1.0.0)

Product number: YC199A01H

1 Introduction, cyclamate is a non-nutritive synthetic sweetener, its sweetness is 30 times that of sucrose, and the price is only one-third of sucrose, if consumers often eat drinks or other foods with excessive cyclamate content, it will cause harm to the liver and nervous system of the human body due to excessive intake, especially for the elderly, pregnant women and children with weak metabolic detoxification ability.

2 Limited standard GB2760-2014 "Food Additive Use Standard" stipulates: the limit standard for beverages, jellies, biscuits, and prepared wines is 0.65g/kg, the limit standard for pickled vegetables, jams, and preserves is 1.0g/kg, and the limit standard for plums, cold fruits, and fruit cakes is 8.0g/kg.

3 Detection principle, after cyclamate reacts with reagents, a fuchsia compound is generated, and the color depth is proportional to the content within a certain range.

4 Detection range, the determination of beverages, biscuits, pastries, preserves and condiments, pickles, etc.

5 Technical indicators, the lower limit of detection: 250 mg/kg, detection range 0-5000 mg/kg.

6 Sample pretreatment

Liquid sample: Shake well and directly absorb 1 mL of the sample in a 5 mL colorimetric tube with a straw, and then add 2 mL of purified water to shake well as the liquid to be tested. (The sample containing carbon dioxide is first heated to remove, and the sample containing alcohol is heated to remove in boiling water.)

Solid sample: such as cold fruit and candied fruit samples, cut them into samples, weigh 1 gram of the sample in a 5 mL colorimetric tube, add 2 mL of purified water to shake and let stand for 5 minutes, and then take 1 mL of the supernatant in the colorimetric tube as the liquid to be tested.

7 Sample determination: Take 1 mL of the liquid to be tested and add it to the 5 mL colorimetric tube, and take another 1 mL of purified water as a blank control.

Add 4 drops of reagent A and 4 drops of reagent B in sequence, shake well and place in the freezer compartment of the refrigerator for 10 minutes, add 1 mL of reagent E immediately after taking it out, shake for 30 seconds, let it stand and stratify, take 0.5 mL of the upper layer solution (if the upper layer is not clarified, centrifuge to clarify the upper layer and absorb it), add 1 mL of reagent F solution, shake vigorously and then let it stand for stratification.

Then absorb the upper layer liquid 200 µL in a 1.5 mL colorimetric tube, add 2 drops of reagent B and 4 drops of reagent C, shake well and add 4 drops of reagent D, observe the results after 5 minutes of color development.

8 Results Determination: If the color of the solution to be tested is significantly darker than the blank control, cyclamate is added to the sample, which can be compared with the color card to obtain the approximate content. If the color is basically the same as the blank control, it indicates that no cyclamate is added.

9 Precautions

9 Use reagents to pay attention to safety, and wash with water immediately after skin contact.

9 Reagent F is a saturated sodium chloride solution, and it is normal to have undissolved crystals.

9 After adding the detection reagent C, shake well and then add reagent D.

9.4 Positive samples need to be re-tested 3 times, and pay attention to compare with the blank control.

9.5 This product is only used for initial screening, and the final result is subject to the relevant national standard methods.

9 Storage conditions and valid period Reagents Store in a cool and dry place at 4-30 ° C, protected from light, and the valid period is 12 months.

10 Kit packing list, specifications: 100 times/box

Name

Quantity

Name

Quantity

Remarks

Reagent A

2 Bottle

Reagent F

1 Bottle

Reagent B

2 Bottle

1. 5 mL Centrifuge Tube

1 Pack

Reagent C

2 Bottle

5 mL Centrifuge Tube

1 Pack

Reuse

Reagent D

2 Bottle