What is the aflatoxin B1 colloidal gold rapid detection card?

Aflatoxin B1 Colloidal Gold Rapid Detection Card is a portable detection tool based on colloidal gold immunochromatography technology for rapid detection of aflatoxin B1 content in food, feed and other samples. It has the characteristics of simple operation, fast detection speed and low cost, and is widely used in on-site rapid screening in food processing, agricultural production, quality supervision and other fields. Core Principle Aflatoxin B1 is a highly toxic and carcinogenic substance produced by fungi such as aflatus. It is commonly found in peanuts, corn, edible oil, feed and other products. The working principle of the colloidal gold rapid detection card is based on the antigen-antibody specific binding reaction:

the detection card is pre-coated with anti-aflatoxin B1 antibodies (fixed in the detection line area) and antibodies for control (fixed in the quality control line area). When the sample to be tested (after pre-treatment to extract aflatoxin B1) is added dropwise to the addition well of the detection card, the aflatoxin B1 in the sample will bind to the colloidal gold-labeled antigen (or antibody), form a complex and move to the other end of the card with the chromatography solution. If the content of aflatoxin B1 in the sample exceeds the detection threshold, it will competitively bind to the antibody, resulting in no color rendering of the detection line; if the content is lower than the threshold, the detection line will develop color due to antibody-antigen binding. The quality control line will develop color regardless of the result, and is used to determine whether the detection card is valid. Key features Fast and efficient: The entire detection process is usually completed within 10-15 minutes, without the need for complex instruments, suitable for on-site immediate detection. Easy to operate: No professional technicians are required, only simple sample pre-processing (such as extraction, dilution) is required, and the sample can be added dropwise according to the instructions to observe the results. Lower cost: Compared with laboratory detection methods such as high-performance liquid chromatography (HPLC), the single-part cost of the detection card is lower, Visualization results: Through the color development of the detection line (T line) and the quality control line (C line), the results (positive/negative) are directly judged without special equipment reading. Application scenario Food industry: Detect whether aflatoxin B1 in peanuts, corn, nuts, edible oil, soy sauce and other foods exceeds the standard to ensure food safety. Feed industry: Screen aflatoxin B1 in feed raw materials (such as soybean meal, corn) and finished products to avoid toxins from entering the food chain through farmed animals. Regulatory department: It is used for on-site rapid testing such as market spot checks and origin quarantine to improve the efficiency of supervision. Precautions The test results are qualitative or semi-quantitative. If accurate values are required, they need to be further confirmed in combination with laboratory instruments (such as HPLC, ELISA). Sample pretreatment needs to be standardized (such as the proportion of extract, purification steps), otherwise it may affect the detection accuracy. The test card needs to be stored at the specified temperature (usually 2-30 ° C) to avoid expiration or moisture, otherwise it will fail.

In conclusion, the aflatoxin B1 colloidal gold rapid test card is a practical on-site screening tool, which can quickly identify high-risk samples and provide preliminary judgment basis for food safety control.