Orange Thiamethione Colloidal Gold Rapid Detection Card Instruction Manual

Product number
: YB083C01K

Product Overview

Thiamethione is a neonicotinoid insecticide that mainly acts on the nervous system of insects. It can selectively bind to nicotinic acetylcholine receptors in insects, block the transmission of nerve signals, and finally kill pests. It has the characteristics of high efficiency, strong internal absorption, long shelf life, and high safety. It is widely used in pest control of citrus fruits such as oranges.

This product is a rapid detection card for colloidal gold of thiamethione special for oranges. It is optimized and upgraded for the special substrate containing essential oils and high sugar content in orange peel, effectively reducing substrate interference and strong detection stability. No large-scale precision instruments are required, the operation is simple, the detection speed is fast, and the on-site primary screening of thiamethione pesticide residues in orange samples can be quickly completed. It is suitable for various scenarios such as orange planting bases, fresh processing sorting, farmers' supermarkets, market supervision and sampling.

Second, the detection principle

This product adopts the principle of competitive inhibition immunochromatography. The binding of thiamethione in the sample to the specific antibody labeled with colloidal gold inhibits the binding of the antibody to the antigen on the NC membrane detection line (T line), resulting in changes in the color depth of the T line. The operator can accurately determine the residual content of thiamethione in the orange sample by comparing the color depth of the T line and the C line.

III. Product use

It is mainly used for rapid qualitative primary screening of thiamethione pesticide residues in fresh orange samples, and is also suitable for screening of thiamethione residues in various fresh vegetables and fruit samples to meet the quality self-inspection of agricultural products, batch shipment quality inspection, market compliance sampling and other testing needs.

IV. Product technical indicators and performance

1. Minimum detection limit for samples

0.01 mg / kg (ppm)

2. Cross-reactivity

This product has excellent detection specificity and strong resistance to similar pesticide interference: the cross-over rate with imidacloprid is about 1.5%; the cross-over rate with furfuran, amidacloprid, thiamidacloprid, and domicillin is less than 0.5%, with almost no cross-interference, which can accurately screen for thiamidacloprid residues in orange samples.

V. Kit composition

This kit is divided into 10 times/box and 20 times/box. The accessories are complete and ready to use. The specific components are as follows:

VI. Precautions

2. Samples should be made of fresh and intact oranges to avoid the use of spoiled samples; clean up the large pieces of soil and dirt attached to the surface of the orange peel to prevent affecting the sample extraction and testing effect.

3. Before testing, it is recommended that the samples be fully stirred and mixed. When sampling, representative parts such as orange pulp and peel are selected for weighing testing to ensure that the test results truly reflect the actual drug residue of the sample.

4. The test environment temperature should be controlled at 17 to 30 degrees Celsius. Excessive or too low temperature will affect the color rendering effect of the test paper, causing deviation in the test results.

5. Please strictly follow the detection steps for testing. Do not touch the color rendering area of the test strip during operation, and avoid direct sunlight and electric fans blowing the test strip directly.

6. The sample solution to be tested should be kept clear and transparent, and the turbidity of the solution can easily lead to inconspicuous color development and abnormal background, which will affect the judgment of the experimental results.

7. Products that have expired or the aluminum foil bag is damaged cannot be used. Please use the test card after unpacking immediately and cannot be stored for a long time.

8. Please complete the test as soon as possible after the sample is processed. If the storage time is too long, the sample needs to be reprocessed before testing.

9. When a positive result appears, it is recommended to retest. The test results of this product are for initial screening reference only. If accurate confirmation is required, it is necessary to refer to the relevant national standard methods for testing and judgment.

10. When adding standards for verification, methanol is generally selected as the solvent for the standard solution, and the final sample addition is recommended to be controlled at 10-50 μL.

11. When directly testing the standard, the solvent needs to be extracted with the kit, and the amount of organic solvent added is controlled within 1%. Tap water, distilled water, purified water or deionized water cannot be used as negative controls.

VII. Safety instructions

1. The experimental operation needs to match the corresponding experimental equipment, and the operator needs to wear white clothes, gloves, masks and other necessary experimental equipment to carry out the testing work in a standardized manner.

2. The test kit should be properly stored and placed in a place that is not easily accessible to children to avoid accidental touch and ingestion.

3. After the experiment, keep the laboratory clean and tidy to ensure air circulation in the experimental environment.

4. This product is a one-time use product and cannot be reused. After the test is completed, the experimental waste needs to be collected separately, and it is recommended to dispose of it according to the medical waste specification.

5. The supporting reagents of this product are safe and reliable, and do not contain carcinogenic, highly toxic, flammable, explosive, and strongly corrosive ingredients, but it is strictly prohibited to eat.

Storage conditions and valid period

1, the original packaging products: 4-30 ℃ in the dark dry storage, prohibit freezing, valid period of 12 months.

2. Product after unpacking: The test card needs to be used immediately after unpacking, and frozen storage is prohibited.