Rapid Detection Card for Colloidal Gold in Orange

Thiamethione Colloidal Gold in Orange Rapid Detection Card Instruction Manual

Product number
: YB083C01K

Product Overview

Thiamethione is a neonicotinoid insecticide that mainly acts on the nervous system of insects. It can selectively bind to nicotinic acetylcholine receptors in insects, block the transmission of nerve signals, and finally kill pests. It has the characteristics of high efficiency, strong internal absorption, long shelf life, and high safety. It is widely used in pest control during the planting of orange fruits.

This product is a special colloidal gold rapid detection card for orange fruit. It is optimized for the special substrate containing oil and high sugar in orange peel, effectively reducing substrate interference, and the detection results are stable and reliable. The product does not require large-scale precision instruments, is simple to operate, and has fast detection speed. It is suitable for on-site rapid initial screening of pesticide residues in orange fruit by orange planting bases, fresh processing enterprises, farmers' supermarkets, and market supervision departments.

Second, the detection principle

This product uses the principle of competitive inhibition immunochromatography. The specific antibody labeled with colloidal gold binds to thiamethione in the sample, which inhibits the binding of the antibody to the antigen on the NC membrane detection line (T line), resulting in changes in the color depth of the T line. The operator can accurately determine the residual content of thiamethione in the orange fruit sample by comparing the color depth of the T line and the C line of the test strip.

III. Product use

It is mainly used for the rapid qualitative primary screening of thiamethione content in fresh oranges and other orange fruit samples, and is also suitable for the screening of thiamethione residues in various fresh vegetables and fruit samples. It can meet the requirements of self-inspection of the place of origin, quality inspection of enterprise warehousing, and sampling inspection of market supervision.

IV. Product technical indicators and performance

1. Minimum detection limit for samples

0.01 mg / kg (ppm)

2. Cross-reactivity

This product has strong detection specificity and excellent anti-interference performance: the crossover rate with imidacloprid is about 1.5%; the crossover rate with furfuran, amidacloprid, thiamethoxam, and domicillin is less than 0.5%, with almost no cross-interference. It can accurately detect thiamethiamine residues in orange fruit samples.

V. Kit composition

This kit is divided into 10 times/box and 20 times/box. The accessories are complete and ready to use. The specific components are as follows:

VI. Precautions

1. Tools such as knives, scissors, and stickers for pre-testing samples should be cleaned to avoid cross-contamination.

2. Samples should be made of fresh and intact orange fruits to avoid spoilage and deterioration of the samples; clean up the large pieces of soil and dirt attached to the surface of the orange fruits to prevent affecting the sample extraction and test results.

3. Before testing, it is recommended that the samples be fully stirred and mixed. When sampling, representative parts such as orange pulp and peel are selected for weighing testing to ensure that the test results truly reflect the actual drug residue of the sample.

4. The test environment temperature should be controlled at 17 to 30 degrees Celsius. Excessive or too low temperature will affect the color rendering of the test paper, resulting in deviations in the test results.

5. Please strictly follow the detection steps for testing. Do not touch the color rendering area of the test strip during operation, and avoid direct sunlight and electric fans blowing the test strip directly.

6. The sample solution to be tested needs to be kept clear. The turbidity of the solution can easily lead to inconspicuous color development and abnormal background, which will affect the judgment of the experimental results.

7. Products that have expired or the aluminum foil bag is damaged cannot be used. Please use the test card immediately after unpacking, and do not store it for spare.

8. Please complete the test as soon as possible after the sample is processed. If the storage time is too long, the sample needs to be reprocessed before testing.

9. When a positive result appears, it is recommended to retest. The test results of this product are for reference only. If you need confirmation, please refer to the relevant national standards and methods.

10. When adding standards for verification, methanol is generally selected as the solvent for the standard solution, and the final sample addition is recommended to be controlled at 10-50 μL.

11. When directly testing the standard solvent, use the extract of the kit, and the amount of organic solvent added is controlled within 1%. Tap water, distilled water, purified water or deionized water cannot be used as a negative control.

VII. Safety instructions

1. The experiment needs to match the corresponding experimental equipment, and the operator needs to wear white clothes, gloves, masks and other necessary experimental equipment to standardize the operation.

2. The test kit should be properly stored and placed in a place that is not easily accessible to children to prevent accidental ingestion.

3. After the experiment, keep the laboratory clean and tidy, and maintain air circulation in the experimental environment.

4. This product is a one-time product. It should be properly disposed of after testing. The experimental waste should be collected separately. It is recommended to dispose of it as medical waste.

5. The reagents involved in this product are safe and reliable, and do not contain carcinogenic, highly toxic, flammable, explosive, and highly corrosive reagents, but are strictly prohibited from consumption.

Storage conditions and valid period

1, the original packaging: 4-30 ℃ in the dark dry storage, do not freeze, valid period of 12 months.

2. After unpacking: Use the test card immediately after unpacking, do not freeze.