Rapid Detection Card for Milk Adulteration in Camel Milk and Goat Milk (Colloidal Gold Method) Instruction Manual

1. Test Principle

Immunochromatographic Competitive Method. During detection, the milk component in the sample binds to the gold-labeled antibody conjugate at the binding pad, thereby inhibiting the binding of the gold-labeled conjugate to the milk component antigen on the solid-phase carrier membrane. If the content of milk components in the sample is greater than the corresponding detection limit, the T-line is not colored, otherwise the T-line is excellent.

2. Intended Use

Camel Milk and Goat Milk Screening of Milk Components

3. Cross-Reaction and Product Performance

Detection Limit: 2%

The detection of the addition of melamine, goat milk or camel milk was negative; the false positive rate of this product was 15%, and the false negative rate

IV. Instrument and equipment required for experiment

Oscillator (optional)

V. Main components

Component name

10 parts/box

Component name

10 parts/box

Camel milk, goat milk adulterated milk Rapid detection card

2 0 parts

Instruction manual

1 parts

6. Storage conditions and valid period

Original packaging: 4-30 ℃ dry storage in the dark, valid period of 12 months.

After opening: Please use the test card within half an hour after opening, do not freeze.

VII. Sample requirements

1. Solid fermented milk and beverages cannot be detected;

2. Samples should be warmed up before testing, and to avoid rancid samples.

Test method

Pre-treatment of milk powder samples

Weigh 0.5g of sample in a 15mL centrifuge tube, add 10mL of purified water, and mix well to form the solution to be tested.

Direct test of fresh milk samples (not finished, please store in a 4-degree refrigerator in time, and complete the test within 24 hours. If the sample is slightly rancid and aggregates, it will lead to incomplete chromatography. You can take the middle layer for testing after centrifugation)

Nine, sample testing

Return the test card to room temperature, and disassemble it and place it flat on the experimental table;

Absorb 100 μL (about 3 drops) of liquid to be tested and add it to the sample hole, and time it for 5 minutes;

Ten, interpretation of test results

Visual test:

negative (not detected): C-line color development, T-line visible to the naked eye, and regardless of color depth;

Positive (detected): C-line color development, T-line color is not visible, or T-line is vague;

Invalid: C-line color is not visible and regardless of whether T- Color.

Instrument interpretation: For details, please refer to the instrument instruction manual

11. Precautions

1. This product is only for qualitative screening. If you need confirmation, please refer to the relevant national standard methods.

2. It is recommended to repeat the verification when encountering positive samples.

3. Do not mix test cards and reagents from different batches, and do not use reagents that exceed the valid period.

4. After the test reagents are taken out of the refrigerator, the temperature should be restored to room temperature before starting the test.

5, the detection card for one-time use, please do not touch the detection card in the center of the white film surface, in the second step of the reaction within 3 minutes to read the results, more than 5 minutes after the interpretation of the results invalid.

6, before the test is recommended to fully stir the sample mixed (if less sample should be taken representative parts, and then weighed), so that the test results can be more realistic reaction sample of the actual drug residue.

7, the reagents involved in this product are safe and reliable, do not contain carcinogenic, highly toxic, flammable, explosive, strong corrosive reagents.

8, the reagents of this product are disposable, the waste after use should be treated as general chemicals.