Wuhan Yupinyan Cypermethrin Colloidal Gold Rapid Detection Card

Cypermethrin Colloidal Gold Quick Detection Card Instruction Manual

Product Number: YPAW-14

1 Principle and Use

This product is made of the principle of competitive inhibition colloidal gold immunochromatography for the detection of cypermethrin pesticide residues in vegetables and fruits. After the sample solution is dropped into the sample hole of the test card, the cypermethrin in the sample solution binds to the gold standard antibody, thereby preventing the gold standard antibody from binding to the cypermethrin conjugate on the cellulose membrane. The detection result is judged by the color depth of the C and T lines.

2 technical indicators, detection limit: 50 mg/kg (ppm)

3 Need to bring your own tools

Electronic balance (0.1-100 g), scissors, forceps, disposable cups, vortex mixer, pipette (0.1-1 ml), timer.

4 sample pretreatment

Take an appropriate amount of sample, cut about 1 cm square, weigh 1 g of shredded sample in the detection tube, add 1 ml of diluent, fully oscillate for 2 minutes, let stand for 1 minute, as the liquid to be tested;

5 sample detection

(1) Tear the aluminum foil packaging bag of the detection card, take out the detection card/gold standard microporous/dropper (a total of 3 kinds of consumables), and put it on a flat, clean table.

(2) with a pipette or aluminum foil bag matching dropper to absorb the above liquid to be tested 120 µL (about 4 drops) in the gold standard micropores, blown with a dropper to completely dissolve the purplish-red material in the pores, let stand horizontally, and wait for the reaction for 2 minutes; all the liquid in the gold standard micropores is added dropwise to the sample hole (S) of the detection card;

(3) start timing after adding the sample, and leave it at room temperature for 8-10 minutes to judge the result, and the other time interpretation is invalid.

6 the result judgment

negative (-): the control C line appears purplish-red line, and the color development of the test T line is consistent with or deeper than the C line, indicating that the concentration of cypermethrin in the sample is lower than the detection limit or there is no cypermethrin residue

Positive (+): In the detection window, the purple-red line appears on the C line, and the T line does not develop color or is lighter than the C line, indicating that the cypermethrin concentration in the sample is higher than the detection limit.

invalid: In the detection window, the purple-red line does not appear on the C line.

7 Precautions

(1) Products that have expired or the aluminum foil bag is damaged should not be used.

(2) When the test card is taken out of the refrigerator, it should be restored to room temperature and opened. The test card after opening should be used as soon as possible to avoid failure.

(3) Do not touch the white film surface in the center of the test card.

(4) The liquid extraction dropper cannot be mixed to avoid cross-contamination.

(5) The liquid to be tested should be clear, free of cloudy particles, and free of bacterial contamination, otherwise it will easily lead to abnormal phenomena such as blockage and inconspicuous color development, which will affect the judgment of experimental results.

(6) Please store the reagent in a place that is not easy for children to contact, and do not eat the equipped reagent.

(7) If you need to directly test the standard product, please prepare it with the diluent provided in the kit.

8 Storage and shelf life

(1) Storage conditions: 4-30 ° C Store in the dark, do not freeze.

(2) Shelf life: 1 year, see the outer packaging for the production date.

9 kit composition

specification

composition

10 times/box

20 times/box

test card (containing gold standard micropores, dropper, desiccant)

10 parts