Thiamethoxam Colloid Gold Quick Detection Card Instruction Manual

Product Number: YB084C01K

Summary

Thiamethoxam (Thiamethoxam) is a high-efficiency and low-toxicity insecticide, which belongs to the second generation of nicotine high-efficiency and low-toxicity insecticides, chemical formula is C8H10CIN5O3S, which has gastric toxicity, contact and internal absorption activity to pests. It is used for foliar spray and soil irrigation. It has good anti-effect on piercing pests such as aphids, planthoppers, leafhoppers, whiteflies, etc.

Detection principle

This product adopts the principle of competitive inhibition immunochromatography. The binding of thiamethoxam in the sample to the specific antibody labeled with colloidal gold inhibits the binding of the antibody to the antigen on the NC membrane detection line (T line), resulting in the change of the color depth of the T line; according to the color depth judgment results of the T line and the C line.

Use

for the initial screening detection of the content of thiamethoxam in fresh green onion samples.

The minimum detection limit of the sample

0 mg/kg (ppm)

Cross-reactivity and product performance

The results of adding 10ppm of thiamethoxifen, imidacloprid, furomefenacloprid and thiamethifen were negative;

The results of adding 0.3ppm of thiamethifen and 0.1ppm of enamethifen were positive

kit composition

serial number

specification
composition

10 times/box

2 0 times/box

(1)

test card (1T/bag)

10 card

20 card

(2)

pesticide residue extract (70mL/bottle)

1 bottle

2 bottle

(3)

disposable straws

10

20

(4)

2 mL centrifuge tube

10

20

(5)

manual

1

1

(1) Knives, scissors, sticky plates and other tools should be cleaned to avoid cross-contamination.

(2) Sample requirements: avoid corruption and deterioration of samples; avoid large pieces of soil (

(3) Before the test, it is recommended that the sample be fully stirred and mixed (if the sample is less, the representative part should be taken, and then the sample should be weighed), so that the test results can more realistically reflect the actual drug residue of the sample.

(4) The test environment temperature should be controlled at 17~ 30 ℃. If the temperature is too high or too low, it will affect the test results.

(5) Please follow the test steps to test. Do not touch the color-developing area of the test strip during operation. Avoid direct sunlight and direct blowing of electric fans.

(6) The solution of the sample to be tested needs to be clarified, otherwise it will easily lead to abnormal phenomena such as inconspicuous color development and affect the judgment of the experimental results.

(7) Products that have expired or damaged aluminum foil bags should not be used. Please use the test card immediately after un

(8) Please use the sample as soon as possible after processing. If the time is too long, you need to re-process the sample for re-testing.

(9) When a positive result appears, re-testing is recommended. The test results of this product are for reference only. If you need to confirm, please refer to the relevant national standard methods.

(10) When adding the standard for verification, the standard solution solvent generally chooses methanol, and the final sample is recommended to add 10-50 μL.

(11) When directly testing the standard, the solvent should be matched with the extract of the kit. The amount of organic solvent added is controlled within 1%. Tap water, distilled water, purified water or deionized water cannot be used as a negative control.

Safety instructions

(1) The experiment should match the corresponding experimental equipment and wear the necessary experimental equipment (white clothes, gloves, masks, etc.).

(2) The test kit should be properly stored, please put it in a place that is not easy for children to come into contact with.

(3) After the experiment, keep the laboratory clean and the air flow of the experimental environment.

(4) This product is a one-time product. After the test is completed, it should be properly disposed of. The experimental waste is collected separately. It is recommended to dispose of it according to the medical waste.

(5) The reagents involved in this product are safe and reliable, and do not contain carcinogenic, highly toxic, flammable, explosive, and highly corrosive reagents, but should not be eaten.

storage conditions and valid period

(1) Original packaging: Dry storage at 2-30 ° C in the dark, do not freeze, valid period 12 months.

(2) After unpacking: Use the test card immediately after unpacking, do not freeze.