What is the Chlorpyrifos Colloidal Gold Rapid Detection Card?

In modern agricultural production, the rational use of pesticides is essential to ensure food security, but some pesticides (such as chlorpyrifos) can pose a potential threat to human health if their residues exceed the standard. In order to quickly and accurately detect chlorpyrifos residues in food, researchers have developed the Chlorpyrifos Colloidal Gold Rapid Detection Card, which is a rapid detection product based on colloidal gold immunochromatography technology.

By definition, the Chlorpyrifos Colloidal Gold Rapid Detection Card combines chlorpyrifos antibodies with colloidal gold particles, and realizes qualitative or semi-quantitative detection of chlorpyrifos residues in samples on test strips through the principle of immune competition reaction. It is mainly composed of sample pad, binding pad, nitrocellulose membrane (NC membrane) and absorbent paper, in which the NC membrane is coated with a detection line and a quality control line, the detection line is coated with chlorpyrifos-BSA conjugate, the quality control line is coated with sheep anti-mouse IgG antibody, and the binding pad is fixed with a colloidal gold-labeled chlorpyrifos monoclonal antibody.

The detection principle is not complicated: when the sample (such as vegetable extract, fruit homogenate, etc.) is added dropwise to the sample hole of the detection card, it will move forward along the test strip, and when it reaches the binding pad, the chlorpyrifos in the sample will bind to the antibody labeled with colloidal gold; continue to move to the detection line area of the NC membrane. If the concentration of chlorpyrifos in the sample is lower than the detection line threshold, the antibody labeled with colloidal gold will bind to the chlorpyrifos-BSA conjugate of the detection line, forming a red band on the detection line. If the concentration of chlorpyrifos in the sample is higher than the threshold, it will competitively bind to the antibody labeled with colloidal gold, resulting in no band in the detection line. The quality control line will always develop color to determine whether the detection card

Compared with traditional detection methods, the Chlorpyrifos Colloid Gold Rapid Detection Card has significant advantages: First, the detection speed is fast, the whole process only takes 10-15 minutes, without the need for complex instruments and equipment; Second, the operation is simple, the user does not need professional training, follow the instructions and steps to complete the detection; Third, the cost is low, suitable for grass-roots regulatory departments, enterprise self-inspection and personal rapid screening use; Fourth, the results are intuitive, the results can be judged by observing the color development of the detection line and the quality control line, and the color development is clear and easy to read.

In the application scenario, it is widely used in the rapid screening of chlorpyrifos residues in agricultural products (such as vegetables, fruits, grains), drinking water, soil and other samples. For example, farmers can quickly detect whether pesticide residues in crops exceed the standard before harvest to avoid food risks; food processing plants can conduct entrance testing of raw materials to ensure the safety of raw materials; market supervision departments can also quickly screen unqualified products in daily sampling inspections to ensure the safety of consumers' dining tables in a timely manner.

However, when using, it is necessary to pay attention to the standardization of sample pre-processing to avoid impurities interfering with the test results; at the same time, the test card needs to be stored at the specified temperature to ensure the activity of reagents. Overall, the chlorpyrifos colloid gold rapid test card has become an important tool in the field of food safety rapid detection with its fast, convenient and efficient characteristics, and plays a key role in safeguarding the safety of public diets.