In the current era of increasing attention to food safety, bean sprouts, as a common ingredient on the public table, have always been concerned about their safety. In the production process of bean sprouts, some unscrupulous merchants may use plant growth regulator sodium 4-chlorophenoxyacetate (a phenoxycarboxylic acid herbicide, which is also a plant growth regulator, and excessive use will cause potential harm to human health) in order to quickly and accurately detect the residue of this substance in bean sprouts, the 4-chlorophenoxyacetate sodium colloidal gold rapid detection card came into being, becoming an important tool to protect the safety of bean sprouts.
So, what is the 4-chlorophenoxyacetate sodium colloidal gold rapid detection card? In simple terms, it is a rapid detection product based on colloidal gold immunochromatography technology, which is mainly used for qualitative or semi-quantitative detection of sodium 4-chlorophenoxyacetate residues in bean sprouts. Its core principle is to use antigen-antibody specific binding reaction: the detection card contains colloidal gold-labeled sodium 4-chlorophenoxyacetate-carrier protein conjugate (ie gold pad), and the detection line (T line) and quality control line (C line) coated with specific antibodies. When the treated bean sprout sample solution is added to the sample well of the detection card, the solution will pass through the gold label pad. If the sample contains sodium 4-chlorophenoxyacetate, it will bind to the colloidal gold-labeled antibody on the gold label pad to prevent it from binding to the antigen on the detection line (T-line); conversely, if the sample does not contain this substance, the colloidal gold-labeled antibody will bind to the T-line antigen and develop color on the T-line and C-line respectively to form two color ribbon.
As a rapid detection tool, the 4-chlorophenoxyacetate sodium colloidal gold rapid detection card has significant advantages. First of all, the detection speed is extremely fast, usually only 10-15 minutes to produce results, much faster than traditional high-performance liquid chromatography and other detection methods, which can meet the needs of on-site rapid screening; secondly, the operation is simple and convenient, without professional experimental equipment and complex pre-processing steps, ordinary personnel can get started with simple training; furthermore, the cost is relatively low, suitable for large-scale promotion and use; at the same time, it has high sensitivity, can detect low-concentration residues, and the result interpretation is intuitive, by observing whether the color ribbon appears to determine whether the sample is qualified.
At present, the test card is widely used in the sampling inspection of bean sprouts in farmers' markets and catering units by market supervision departments, the self-inspection of raw materials in food production enterprises, and the experimental research of scientific research institutions. Its appearance not only provides efficient and practical technical support for bean sprouts safety supervision, but also adds a guarantee to consumers' "vegetable basket" safety.