Rapid Detection Card for Thiamethione Colloidal Gold in Drupe Fruits

Thiamethione Colloidal Gold Rapid Detection Card in Drupe Fruits Instruction Manual

Product number
: YB083C01K

Product Overview

Thiamethione is a neonicotinoid insecticide that mainly acts on the nervous system of insects. It can selectively bind to the nicotinic acetylcholine receptor in insects, block the transmission of nerve signals, and finally kill pests. It has the characteristics of high insecticidal efficiency, strong crop absorption, long effective period, and high application safety. It is widely used in peaches, plums, apricots, cherries and other stone fruit pest control.

This product is a rapid detection kit for stone fruit. It is optimized for the characteristics of the pulp matrix of stone fruit. It adopts mature colloidal gold immunochromatography technology, which is simple to operate and fast to detect. It does not require large instruments. It can quickly screen for thiamide pesticide residues in stone fruit on site. It is suitable for various scenarios such as orchards, fresh food enterprises, supermarkets and regulatory sampling.

Second, the detection principle

This product uses
Principle of Competitive Inhibition Immunochromatography
Carry out detection. Thiamethione in the sample will bind to the specific antibody labeled with colloidal gold, inhibit the binding of the antibody to the antigen on the NC membrane detection line (T line), and then change the color development depth of the detection line. The operator can determine the color development depth of the sample by comparing the T line of the test strip with the control line (C line).

III. Product use

Suitable for peach, plum, apricot, cherry and other fresh fruit samples of thiamethione residues in the rapid qualitative screening, while all kinds of fresh vegetables, fruits thiamethione residue screening, to meet the self-inspection of agricultural products, batch quality inspection, market supervision sampling and other testing needs.

IV. Product technical indicators and performance

1. Minimum detection limit for samples

0.01 mg / kg (ppm)

2. Cross-reactivity

This product has excellent detection specificity and strong anti-interference ability: the crossover rate with imidacloprid is about 1.5%; the crossover rate with furfuran, amidacloprid, thiamethoxam, and polymycin is less than 0.5%, effectively avoiding the interference of similar pesticides and ensuring accurate and stable detection results.

V. Kit composition

This kit is divided into 10 times/box and 20 times/box. The accessories are complete and ready to use. The specific components are as follows:

VI. Precautions

1. Tools such as knives, scissors, and stickers for pre-testing samples should be cleaned to avoid cross-contamination.

2. It is strictly forbidden to use spoiled samples for testing. Samples of stone fruits must be kept fresh and intact. Clean up impurities and dirt attached to the surface of the sample to avoid affecting the test results.

3. Before testing, it is recommended that the sample be fully stirred and mixed, and the representative parts of the fruit be selected for sampling and weighing during sampling to ensure that the test results truly reflect the actual drug residue of the sample.

4. The detection environment temperature needs to be controlled at 17 to 30 degrees Celsius. Excessive or too low temperature will affect the color rendering effect of the test paper, resulting in deviations in the test results.

5. Test in strict accordance with the standard testing steps. Do not touch the color-rendering area of the test strip during operation. Avoid direct sunlight and electric fans blowing the test strip directly during the testing process.

6. The sample solution to be tested needs to be kept clear and transparent, and the turbidity of the solution can easily lead to problems such as inconspicuous color rendering and abnormal background, which will affect the judgment of the experimental results.

7. Products that have expired or the aluminum foil bag is damaged are prohibited from being used. The test card after unpacking should be used immediately and cannot be stored for backup.

8. After the sample is processed, the test needs to be completed as soon as possible. If the placement time is too long, the sample needs to be reprocessed before testing.

9. When the test has a positive result, it is recommended to retest. This product is only used for rapid initial screening, and the test results are for reference only. If accurate confirmation is required, it is necessary to refer to the relevant national standard methods for testing and judgment.

10. When adding standards for verification, methanol is generally selected as the solvent for the standard solution, and the final sample addition is recommended to be controlled at 10-50 μL.

11. When directly testing the standard, the solvent needs to be extracted with the kit, and the amount of organic solvent added is controlled within 1%. Tap water, distilled water, purified water or deionized water cannot be used as negative controls.

VII. Safety instructions

1. The experimental operation needs to match the corresponding experimental equipment, and the operator needs to wear white clothes, gloves, masks and other necessary experimental equipment to carry out the testing work in a standardized manner.

2. The test kit should be properly stored and placed in a place that is not easily accessible to children to avoid accidental touch and ingestion.

3. After the experiment, keep the laboratory clean and tidy to ensure air circulation in the experimental environment.

4. This product is a one-time use product and cannot be reused. After the test is completed, the experimental waste needs to be collected separately, and it is recommended to dispose of it according to the medical waste specification.

5. The supporting reagents of this product are safe and reliable, and do not contain carcinogenic, highly toxic, flammable, explosive, and strongly corrosive ingredients, but it is strictly prohibited to eat.

Storage conditions and valid period

1. Original packaged products: dry and stored at 4-30 ° C away from light, no freezing, the valid period of the product is 12 months.

2. Product after unpacking: The test card needs to be used immediately after unpacking, and frozen storage is prohibited.