Thiamethione Colloidal Gold Rapid Detection Card in Ginger and Other Root Vegetables Instruction Manual
Product number
: YB083C01K
Product Overview
Thiamethione is a neonicotinoid insecticide that mainly acts on the nervous system of insects. It can selectively bind to the nicotinic acetylcholine receptor in insects, block the transmission of nerve signals, and finally kill pests. It has the characteristics of high efficiency, strong internal absorption, long shelf life, and high safety. It is widely used in pest control of ginger, potatoes, sweet potatoes and other root vegetables.
This product is a rapid detection card for clothiamide colloidal gold for rhizome vegetables. It is optimized for ginger, potato and other rhizome substrates, and can quickly complete the on-site rapid screening of thiamide residues in samples. It is easy to operate and fast to detect. It is suitable for batch screening and daily self-inspection of various rhizome vegetables.
Second, the detection principle
This product adopts the principle of competitive inhibition immunochromatography. The binding of thiamethione in the sample to the specific antibody labeled with colloidal gold inhibits the binding of the antibody to the antigen on the NC membrane detection line (T line), resulting in the change of the color depth of the T line. By comparing the color depth of the T line and the C line, the residual content of thiamethione in the sample can be determined.
III. Product use
It is mainly used for rapid screening of thiamethione residues in various fresh root vegetable samples such as ginger, sweet potatoes, potatoes, etc. At the same time, it is suitable for rapid detection of thiamethione residues in conventional fresh vegetable and fruit samples, and is suitable for planting bases, processing plants, supermarkets, market supervision on-site sampling and other scenarios.
IV. Technical indicators and product performance
1. Minimum detection limit for samples
0.01 mg / kg (ppm)
2. Cross-reactivity
This product has good specificity and strong anti-interference ability: the crossover rate with imidacloprid is about 1.5%; the crossover rate with furfuran, amidacloprid, thiamethoxam, and polymycin is less than 0.5%, which can effectively avoid the interference of similar pesticides.
V. Kit composition
This kit is divided into 10 times/box and 20 times/box. It is fully equipped and ready to use. The specific components are as follows:
serial number | composition | 10 times/box | 20 times/box |
|---|---|---|---|
1 | Test card (1T/individually packaged) | 10 Card | 20 cards |
2 | Pesticide residue extract (100 mL/bottle) | 1 bottle | 2 bottles |
3 | 2 mL centrifuge tube | 10 sticks | 20 sticks |
4 | instruction manual | 1 serving | 1 serving |
VI. Precautions
1. Tools such as knives, scissors, and stickers for pre-testing samples should be cleaned to avoid cross-contamination.
2. Samples should be fresh and intact, and it is forbidden to use spoiled and deteriorated samples; large pieces of soil attached to the surface of rhizome samples should be thrown off or wiped with clean items to avoid affecting the extraction effect.
3. Before testing, it is recommended that the samples be fully stirred and mixed, and the representative parts of rhizome vegetables are selected for weighing and testing during sampling to ensure that the test results truly reflect the actual drug residue of the samples.
4. The test environment temperature should be controlled at 17 to 30 degrees Celsius. Excessive or too low temperature will directly affect the color rendering of the test paper and the accuracy of the test results.
5. Test strictly according to the detection steps. Do not touch the color-rendering area of the test strip during operation. Avoid direct sunlight and electric fans blowing the test strip during the detection process.
6. The sample solution to be tested needs to be kept clear, and the turbidity of the solution can easily cause inconspicuous color development and abnormal background, resulting in misjudgment of the results.
7. Expired products or products with damaged aluminum foil bags cannot be used. Please use the test card after unpacking immediately and do not keep it for a long time.
8. Please complete the test as soon as possible after the sample is processed. If the storage time is too long, the sample needs to be reprocessed before testing.
9. When a positive result appears, it is recommended to retest. The test results of this product are only used as a reference for rapid initial screening. If accurate confirmation is required, it needs to be tested with reference to relevant national standards and methods.
10. When adding standards for verification, methanol is generally selected as the solvent for the standard solution, and the final sample addition is recommended to be controlled at 10-50 μL.
11. When directly testing standards, the solvent should be used with the kit's supporting extract, and the amount of organic solvent added should be controlled within 1%; tap water, distilled water, purified water or deionized water should not be used as a negative control.
VII. Safety instructions
1. The experimental operation needs to be equipped with corresponding experimental equipment, and the operator needs to wear white clothes, gloves, masks and other necessary experimental equipment to standardize the operation.
2. The test kit should be properly stored and placed in a place that is not easily accessible to children to prevent accidental ingestion.
3. After the experiment, keep the experimental table clean and maintain air circulation in the experimental environment.
4. This product is a one-time use product and cannot be reused. After the test is completed, the experimental waste needs to be collected separately, and it is recommended to dispose of it according to the medical waste specification.
5. The supporting reagents of this product are safe and reliable, and do not contain carcinogenic, highly toxic, flammable, explosive, and strongly corrosive ingredients, but it is strictly prohibited to eat.
Storage conditions and valid period
1. Original packaged products: stored in 4~ 30 ° C dark and dry conditions, prohibited from freezing, valid period of 12 months.
2. Product after unpacking: The test card needs to be used immediately after unpacking, and frozen storage is prohibited.
