Rapid Detection Card for Colloidal Gold in Garlic

Thiamethione Colloidal Gold in Garlic Rapid Detection Card Instruction Manual

Product number
: YB083C01K

Product Overview

Thiamethione is a neonicotinoid insecticide that mainly acts on the nervous system of insects. It can selectively bind to nicotinic acetylcholine receptors in insects, block the transmission of nerve signals, and finally kill pests. It has the characteristics of high efficiency, strong internal absorption, long shelf life, and high safety. It is widely used in pest control during garlic planting.

This product is a garlic-specific thiamethione colloidal gold rapid detection card. It is optimized and upgraded for garlic spicy and high-fiber special substrates, effectively resisting sample matrix interference, and has strong detection stability. No large-scale precision instruments are required, the operation is simple, the detection speed is fast, and the on-site rapid primary screening of thiamethione pesticide residues in garlic samples can be quickly completed. It is suitable for garlic planting bases, fresh processing plants, farmers' supermarkets, market supervision sampling and other scenarios.

Second, the detection principle

This product uses
Principle of Competitive Inhibition Immunochromatography
Detection. Thiamethione in the sample will bind to the specific antibody labeled with colloidal gold, inhibit the binding of the antibody to the antigen on the NC membrane detection line (T line), and then cause the color depth of the T line to change. The operator can visually judge the residue of thiamethione in the garlic sample by comparing the color depth of the T line and the C line of the test strip.

III. Product use

It is mainly used for the rapid qualitative initial screening of thiamethione pesticide residues in fresh garlic samples, and is suitable for the screening of thiamethione residues in various fresh vegetables and fruits to meet the requirements of garlic origin self-inspection, batch shipment quality inspection, market compliance sampling and other testing needs.

IV. Product technical indicators and performance

1. Minimum detection limit for samples

0.01 mg / kg (ppm)

2. Cross-reactivity

This product has excellent detection specificity and strong resistance to similar pesticide interference: the cross-over rate with imidacloprid is about 1.5%; the cross-over rate with furfuran, amidacloprid, thiamethoxam, and domicillin is less than 0.5%, which can effectively avoid cross-interference and ensure the accuracy and reliability of garlic sample detection results.

V. Kit composition

This kit is divided into 10 times/box and 20 times/box. The accessories are complete and ready to use. The specific components are as follows:

VI. Precautions

1. Tools such as knives, scissors, and stickers for pre-testing samples should be cleaned to avoid cross-contamination.

2. Samples should be made of fresh and intact garlic to avoid the use of spoiled and deteriorated samples; large pieces of soil impurities attached to the garlic surface should be cleaned to prevent sample extraction and detection effects from being affected.

3. Before testing, it is recommended that the sample be fully stirred and mixed. When sampling, select the representative parts of garlic for weighing and testing to ensure that the test results truly reflect the actual drug residue of the sample.

4. The detection environment temperature needs to be controlled at 17 to 30 degrees Celsius. Excessive or too low temperature will directly affect the color rendering effect of the test paper, resulting in deviations in the test results.

5. Test strictly according to the detection steps. Do not touch the color-rendering area of the test strip during operation. Avoid direct sunlight and electric fans blowing the test strip during the detection process.

6. The sample solution to be tested needs to be kept clear and transparent, and the turbidity of the solution can easily cause inconspicuous color development and abnormal background, which in turn affects the judgment of the experimental results.

7. Products that have expired or the aluminum foil bag is damaged cannot be used. Please use the test card after unpacking immediately and cannot be stored for a long time.

8. Please complete the test as soon as possible after the sample is processed. If the storage time is too long, the sample needs to be reprocessed before testing.

9. When a positive result appears, it is recommended to retest. The test results of this product are for initial screening reference only. If accurate confirmation is required, it is necessary to refer to the relevant national standard methods for testing and judgment.

10. When adding standards for verification, methanol is generally selected as the solvent for the standard solution, and the final sample addition is recommended to be controlled at 10-50 μL.

11. When directly testing the standard, the solvent needs to be extracted with the kit, and the amount of organic solvent added is controlled within 1%. Tap water, distilled water, purified water or deionized water cannot be used as negative controls.

VII. Safety instructions

1. The experimental operation needs to match the corresponding experimental equipment, and the operator needs to wear white clothes, gloves, masks and other necessary experimental equipment to carry out the testing work in a standardized manner.

2. The test kit should be properly stored and placed in a place that is not easily accessible to children to avoid accidental touch and ingestion.

3. After the experiment, keep the laboratory clean and tidy to ensure air circulation in the experimental environment.

4. This product is a one-time use product and cannot be reused. After the test is completed, the experimental waste needs to be collected separately, and it is recommended to dispose of it according to the medical waste specification.

5. The supporting reagents of this product are safe and reliable, and do not contain carcinogenic, highly toxic, flammable, explosive, and strongly corrosive ingredients, but it is strictly prohibited to eat.

Storage conditions and valid period

1. Original packaged products: dry and stored at 4-30 ° C away from light, no freezing, the valid period of the product is 12 months.

2. Product after unpacking: The test card needs to be used immediately after unpacking, and frozen storage is prohibited.