Azithromycin Rapid Detection Card in Tissue (Colloidal Gold Method) Instruction Manual

Azithromycin Rapid Detection Card in Tissue (Microporous Colorimetry).pdf

Azithromycin in tissue
Quick test card (colloidal gold method) instruction manual

                                                      
Product number:
YB033R01K

One.
summary

Azithromycin (
Cephradine
，
Velosef
), aliases: erythromycin, azithromycin, etc., is a macrolide antibiotic that is resistant to Staphylococcus aureus and a variety of other antibiotic-resistant bacteria
Bacillus
It has a rapid and reliable bactericidal effect. This product is suitable for rapid on-site testing of various types such as breeding and processing enterprises, testing institutions, supervision and management departments, etc.

II.
Detection principle

This product adopts the principle of competitive inhibition immunochromatography. The azithromycin in the sample binds to the specific antibody labeled with colloidal gold, inhibiting the antibody and
NC
Membrane detection line (
T
The antigen on the line) binds, resulting in a change in the color depth of the detection line. Whether or not the sample contains
Azithromycin
The quality control line (
C
Line) will be colored to show that the detection is valid.

three
Intended use

Pork, chicken, fish and aquatic tissue
Class sample initial screening to ensure
Azithromycin
The content is not higher than
Product detection limit
。

Samples tested: pork, beef, chicken, bullfrog, eel, grass carp, silver carp, crucian carp, sea bass, large and small yellow croaker.

IV.
Cross-reaction and product performance

    
检出限：2 m
g/kg
（
ppb
）

detection add
10 ppm
The results of sulfonamides, tetracyclines, amide alcohols, nitrofurans and malachite green were negative.

five
The main components

six
Storage conditions and valid period

Original packaging:
4-
30℃
Dry away from light
Save, valid period
12Month.

After opening: After the test card is opened, please
use immediately
Do not freeze.

seven
Sample requirements

1、
Avoid spoilage of samples.

2Tissue samples are peeled, viscera, fat, blood, bone, shell, etc. For parts like pork and fish, try to extract refined meat to avoid fat sample interference;

3The sample should be fully homogenized.

eight
Test method

Sample pretreatment

1、
say
take
10-50 
gRepresentative sample,
homogenate
weigh
3±0.05 g
Sample at
15milliliter
Inside the centrifuge tube, add
1 mL
Azithromycin Extract
A
，
Join again
4 mL
Azithromycin Extract
B
，
Close the lid.
Vortex oscillation or manual up and down oscillation
1minute
，
4000 rpm
centrifugal
2minute
，
take
2 mL
Blow dry the supernatant;

2、
join
300m
L
Azithromycin complex solution, fully shaken, is
liquid to be tested
。

nine
Sample
detect

1Remove the required number of gold label micropores and add
100m
L
The liquid to be tested,
Suction up and down
4-5
次混匀，
exist
20～40℃开始第一步反should
And time
3minute
；

2、Remove the detection card
，
lay flat
On the desktop
Gold Label
All the liquid in the microwell is transferred to the sample well and timed.
6minute
。
（
Note: After the timing is over, please
3minute
The result is read within, and the interpretation is invalid at other times.
）

ten
Interpretation of test results

Visual inspection:

Negative (not detected):
T
线颜色than
C
The line color is dark or the same.
；

Positive (detected):
T
线颜色than
C
Shallow line or
T
No color rendering

Invalid:
C
线不显色且无论T线是否显色。

Instrument interpretation: See the instrument instruction manual for details

ten
one
Precautions

1This product is for
Qualitative screening
For confirmation, please refer to the relevant national standards and methods.

2When encountering a positive sample, it is recommended to repeat the verification.
 

3、
Tools such as knives, stickers, homogenizers, etc. that process samples before testing should be cleaned to avoid cross-contamination.

4、
The detection temperature should be controlled at
17-30
Degree, too high or too low will affect the test results.

5Before testing, it is recommended that the sample be fully stirred and mixed (if the sample is small, a representative part should be taken, and then the sample should be weighed), so that the test results can more accurately reflect the actual drug residue of the sample.

6、
The solvent of the test standard solution is the extract solution, and water and organic solvents must not be used as negative controls.

7Methanol is generally selected for the liquid solvent during the verification of the standard, and the final sample is recommended to be added at
10-50
m
L
。

8The reagent of this product is a disposable product, and the waste after use should be
General Chemistry
medicine
process
。

9The market-related products are complicated, the quality and performance of each manufacturer are different, and it is not recommended to use congeneric products for Horizontal comparison.