Malachite green immunocolloid gold rapid detection reagent card instruction manual (II nitrogen-free blowing method)

YB136R01K-孔雀石绿_组织_0.5ppb_卡壳_YX60108.docx

                                 Instructions for use of malachite green immune colloid gold rapid test test card

Product number:YB136R01K

1 Principle and use

Malachite green quick test paper Apply the principle of competitive inhibition of immunochromatography Made of, in the sample Malachite green In the process of flow Binding with specific monoclonal antibodies marked with colloidal gold inhibits the binding of the antibody and the malachite green conjunction on the NC membrane detection line (T line), thus leading to a change in the color of the detection line.This product is used for qualitative detection of malachite green residues in fish meat, shrimp meat and other aquatic tissues. The whole testing process only takes 30 minutes.

2 Technical indicators

Detection limit:0.5 Μg/kg (ppb)

3You need to bring your own equipment and reagents

Homogenizer/Scissors, balance, tweezers,Centrifuge,15 mL centrifuge tube,Vortex, pipette, timer

4 Sample pre-processing

The tissue sample (the shrimp should be thoroughly cleaned after the turn and shell, and the fish should be cleaned after the scales) should be refrigerated away from light.

（1）Take peeled and fat removed tissue samples and homogenose them in the homogenizer;

(2) Take3.0 G±0.1 G grams of  homogenised sample in a 15 mL centrifuge tube;

(3) Join in order1Tube reagentA,5 mL reagent B, cover the lid and oscillate for 2 minutes,4000Turn/minute centrifuge for 3 minutes;

(4) Absorb the upper layer of clarification solution 3 Milliliters Up to a clean 5 mL centrifuge tubeMiddle;

(5)SuccessivelyAdd30Zero μLReagentCentigrade, 50 μL Reagent D, Vortex oscillation30Seconds,4000Turn/minute centrifuge1Minute;

(6) Take the lower liquid after centrifugationOne hundred μLAt1.5 MillilitersBe at odds with the community or the leadershipIn the tube (absolutely avoid taking the upper liquid,It is recommended to remove the upper liquid before removing the liquid.), join200 μLReagentE, shockMix well30After a second, asLiquid to be tested.

*Note: After the sample is extracted, please 10 Detection within minutes!

5 Sample testing

 (1) Take out the test card and disposable dropper from the packaging bag;

(2) Use a pipette to suck the above samples before processingMeasureLiquid 100  µL is in the sampling hole (S), and the timing starts after sampling;

(3) The result should be in8-10 Read at the time of the minute, and the reading of the results at other times is invalid;

(4) When reading the results, place the test card horizontally on the front of the observer, and do not turn the test card upside down.

6Result judgment

Negative(-):T line (test line, near the end of the sample hole) color developmentDeepAtPerhapsBe equal toThe color of the C line (control line) indicates that the concentration of the object to be tested in the sample is below the detection limit or there is no residue of the object to be tested.

Positive(Plus):The color rendering of the T line is lower thanCentigradeLine color development, orTIf the line is not colored, it means that the concentration of the test in the sample is higher than the detection limit; the lighter the T line than the C line, the higher the concentration of the test in the sample.If the T line is clearly displayed as green, it can be judged as positive..

Be invalid:Contrast line (C)Not yetA purple stripe appears, indicating that the operation process is incorrect or the detection card has been invalidated. In this case, it should be againCarefulRead the instruction manual and use the new test card.RepeatNew test.If the problem persists, do it immediately.StopStop using this batch number of products and contact the supplier..

7 YXComposition of the kit

8Notes

(1) Please follow the operation steps to test. Do not touch the color display area of the test strip during operation, and avoid direct sunlight when testing.Blow directly with the electric fan.

(2) This test paper is a disposable product. Please do not reuse it.Or mix inspection cards from different batches.

(3)When there is a positive result, it is recommended to retest with this card.The test results of this product are for reference only. If you need to confirm, please refer to the relevant national standards and methods.

(4) If you need to directly test the standard product, you need to use the special diluent in the kit to make it.

(5) Please use it as soon as possible after the sample is processed, and the time process needs to be reprocessed before testing.

(6) Self-water, distilled water, pure water or deionized water cannot be used as negative controls.

9Storage and preservation period

Storage conditions: 4-30℃ away from light, do not freeze.

Warranty period:Valid for 1 year, the production date can be found on the outer packaging.