五氯酚酸钠快速检测卡使用说明书

YB137R01K-五氯酚酸钠_组织_1ppb_微孔_氮吹_2+3_KJ50728.doc

                                 Sodium pentachlorophenolateInstructions for use of the quick test card

                                     Product number: YB137R01K

[Project Overview]

Sodium pentachlorophenolateIt is an organochlorine pesticide, which is often used as a herbicide or bactericide. It is stipulated in the List of Drugs and Other Compounds Prohibited in Food and Animals (Announcement No. 250 of the Ministry of Agriculture and Rural Affairs) released by China in 2019 thatSodium pentachlorophenolateDrugs that are prohibited from being used in food animals shall not be detected in animal food.. Sodium pentachlorophenolateIt decomposes into pentachlorophenol through the food chain. Pentachlorophenol has the toxicity of organic chlorine and phenol, which can inhibit oxidative phosphorylation in the process of biological metabolism, and can cause damage to the liver, kidney and central nervous system of the human body.

[Detection principle]

This product adopts the principle of competitive inhibition immunochromatography.Sample productObjects to be inspectedOrganic reagent extraction, after concentration and redissolution, andMarkedSpecific antibody binding inhibits antibodies and antigens on the detection line (T line) in the detection card.—The combination of BSA couplings leads to changes in the color of the detection line. Qualitative judgment is made by comparing the color of the detection line and the quality control line (C line).

 [Scope of application]

Applicable to Animal food OrganizeIn the sample Sodium pentachlorophenolate Residual Determine the nature of Test.

[Check Go out Limit] 1.0 Μg/kg

 [Testing time] Single sampleTesting time: 28min

[KJProduct composition ] Specification: 10 times/box

[Assist Equipment and facilities]

Scissors, vortex,HomogeneousTrigger mechanism, electronic scale, centrifuge, nitrogen blower or air blow dryer, pipette, timer, 15 Milliliters Centrifuge tube, 5 Milliliters Centrifuge tube, etc.

[Sample bookMove forwardDeal with]

1、Remove fat from animal food and take the edible part of the tissue, and homogenize the sample with a homogenizer;

2.Weigh2±0.1GenerationHomogeneousSample bookIn 15 mL centrifuge tubeMiddle.

3,FaceAbove-mentioned15 Milliliters Add to the centrifuge tube3 Milliliters Extractant,Tighten the centrifuge tube cover,After mixing the sample, time it,By means of120 times/minTaxiFrequency,Violent oscillationOne minute,At room temperature4,000r/MinCentrifugal 3Min;

4. Move with a pipetteTakeOne milliliterSupernatant liquidAt5 Milliliters Centrifuge tubeCentre,

65℃Low position or rank, blow dry with nitrogen blower (or air blow dryer).(Remarks: If the supernatant is not enough after centrifugation, the substance in the centrifuge tube can be shaken and shaken.TenS,4,000r/MinThe above is decentric.3Min)

5,Blow dry5 Milliliters Centrifuge tubeCentreAdd0.3 Milliliters Be used for a special purposeDilution,With 60 times/minTaxiFrequency,Vibrate Mix wellThirty s,ThisIn the centrifuge tubeMixLiquidThat isLiquid to be tested. ( Remarks: The liquid to be tested is as much as possible.30Test within minutes)

[ExamineSteps]

1.ExamineRead the instruction manual completely before use, and check the card before use.,Place gold standard micropores, supporting solutions, etc.To room temperature (20℃-30℃).

2. Take it out of the aluminum foil bagInspection card/gold mark micropore/dropper(A total of three kinds of consumables), horizontally placed in front of the observer.

3. Use a pipette to moveTakeAbove-mentionedIn the pre-processing stepLiquid to be tested120Slightly rise inGold standard microporesIn, useCoordinateDropperGentlePlay wind and percussion instruments30 seconds,Completely dissolvedJin BiaoweiHoleBottomRedSubstance,The water is calm,Waiting for the reaction3Minute,DrawJin BiaoweiInside the holeRedSolution,After gently blowing for 10 seconds, allAdd it toExamineCalorieAdd a sample hole (Small size), start timing after adding the sample.

4.The result should be in 5-8 MinuteInteriorRead,Other time resultsThe judgment is invalid.

[Result judgment]

Yin sex (-):Two purple stripes appeared.TLine (test line, close to one end of the sampling hole) color rendering ratioCentigradeThe line (control line) is deep or as deep, indicating that in the sampleSodium pentachlorophenolateThe concentration is lower than1.0ΜG/kgOr noSodium pentachlorophenolateResidual.

Yang (+):TLine color rendering ratioCentigradeThe line is shallow orTThe line has no color development, indicating that in the sampleSodium pentachlorophenolateHigh residual concentrationAt1.0ΜG/kg,TLine ratioCentigradeThe shallower the line, the more in the sampleSodium pentachlorophenolateThe higher the residual concentration.

Ineffective:Quality control area (Centigrade) There is no purple stripe, indicating that the operation process is incorrect or the detection card has been invalidated.In this case, you should read the manual carefully again and retest with a new test card. If the problem persists, you should immediately stop using this batch numbered product and contact the local supplier.

[Notes]

1. ExamineShellfish are prone to dildos..

2. Pay attention to ensure the freshness of the sample., pay attention to avoid failure or pollution caused by deterioration. When there is a positive result, the sample should be bottled according to the statutory procedure for confirmation testing.

3. Please use the test card once within the shelf life, and avoid direct sunlight and direct blowing of electric fans when testing.

4. In order to ensureThe accuracy of the experimental results. Before testing, the test card and the sample solution to be tested must be restored to room temperature (20℃-3Zero℃) between.

5. Do not touch the white membrane in the center of the card, and do not reuse the equipped dropper to avoid cross-contamination.

6. Tap water, distilled water or deionized water cannot be used as a negative control.

7. Before each test, the props used for cutting and crushing samples must be cleaned to avoid cross-contamination.

8. After the sample is processed, it should be used within 30 minutes. If the time is too long, the sample needs to be reprocessed and then tested.

9. The extractant is a volatile organic solvent. When the temperature is high, the solvent will decrease.

10. This product is for qualitative screening. If you need to confirm, please refer to the relevant national standards and methods.

[Safety Instructions]

 （1）This product does not contain carcinogenic, highly toxic, explosive or highly corrosive reagents, extractantsBelong toFlammable substances.

 （2）During the experiment, you need to wear the necessary laboratory clothes, masks, gloves, etc.Operate.

   （3）TestAfter the end, the used glass reagent bottles and plastic reagent bottles should be rinsed with tap water inside and outside, and the glass bottles and plastic bottles should be placed separately in the designated special recycling bag.,After the garbage collection bag is full, notify the external environmental protection vehicle to recycle and dispose of waste according to the garbage classification.

 （4）The extractant is a volatile organic reagent, and the experimental operation needs to be carried out in a ventilated environment when used.Skin contact:Take off the contaminated clothes and rinse the skin thoroughly with soap and water.Eye contact:Lift the eyelids and rinse them with running water or physiological saline.,Seee medical treatment.

 （5）The extractant is a flammable substance and is not used.It is necessary to close the bottle tightly and keep it closed, and stay away fromTinderAnd heat sources, it is forbidden to use lighters, etc. during use.ChangeTools that generate sparks; SafetyGo outStockpile:StockpileIn a cool and ventilated place, it should be with oxidizing agent, acidAlkaliClass separately stored; in case of fire, use foam, carbon dioxide, dry powder,Sand and soilExtinguish a fire.

[ProductSpecificity]

Use this product to detect the content of100μg/kg hexachloroThe results of residual samples such as phenol, pentachlorobenzene, hexamethylamine, etc. were all negative.

[Storage conditions]   2 ℃~30℃Avoid light at normal temperatureDry storage, do not freeze it.