Instructions for use of saccharin sodium quick test kit in food

Product number: BN-GTE

execution standard: According to GB/T5009.28-2003 detection method

1. Method principle

The saccharin sodium in food reacts with the color developer to form a blue complex. The color depth is proportional to the content of saccharin sodium in the sample. The control analysis software can determine the content of saccharin sodium in food.

2. Scope of application

This kit is suitable for rapid screening of artificial illegal addition of saccharin sodium in food. Three, kit composition, specifications: 10 times/box

serial number

name

quantity

Remarks

1

reagent A

1 bottle

2

reagent B

1 bottle

3

extractant

1 bottle

4

test card

10 card

5

15 mL centrifuge tube

5

can be used repeatedly after cleaning

6

5 mL centrifuge tube

10

test tube

7

1 mL centrifuge tube

5

wine sample purification tube

(can be used repeatedly)

8

1 mL disposable straw

10

9

manual

1

four, sample determination

solid sample:

1, weigh the minced or shredded sample 1.0 g in 15 mL centrifuge tube, add about 9 mL of pure water Add 0.5 mL of the upper liquid to the detection tube, add 1 drop of reagent A and 1 mL of extractant, mix upside down for 20 times, suck out and discard all the upper liquid with a straw, add 2 mL of pure water to the detection tube, 3 drops of reagent B, mix upside down for 20 times, after the liquid is layered, all the upper liquid is sucked out and discarded, and 1-2 drops of the lower liquid are removed in the test area of the detection card, and the analysis software is used for detection.

Liquid sample (non-alcoholic):

Take 0.5 mL of the liquid to be tested in the detection tube with a disposable straw, add 1 drop of reagent A and 1 mL of extractant, mix upside down 20 times, suck out and discard all the upper liquid with a straw, add 2 mL of pure water to the detection tube, 3 drops of reagent B, mix upside down 20 times, after the liquid is layered, all the upper liquid is sucked out and discarded, remove 1-2 drops of the lower liquid in the test area of the detection card, and use the analysis software for detection.

Liquid sample (containing alcohol):

Take 0.5 mL of the liquid to be tested in the wine sample purification tube with a disposable straw, add 1 mL of extractant, and mix upside down 10 times.

Absorb all the upper liquid into the detection tube, add 1 drop of reagent A, 1 mL of extractant, mix upside down for 20 times, suck out and discard all the upper liquid with a straw, add 2 mL of pure water to the detection tube, 3 drops of reagent B, mix upside down for 20 times, after the liquid is layered, all the upper liquid is sucked out and discarded, remove 1-2 drops of the lower liquid into the test area of the detection card, and use analytical software for detection.

5. Detection limit

The detection limit of saccharin sodium in this kit is 5 mg/kg.

Six, precautions

1, the entire testing process should be completed within 2 minutes, otherwise the result is invalid, the instrument parameters must be set before testing;

2, the kit used in the centrifuge tube and other consumables please directly rinse with water, can not be washed with washing powder, detergent and other detergents.

Seven, storage conditions and product valid period

1, storage conditions: cool and dry place storage

2, product valid period: 1 year