Furaltadone Metabolite Colloidal Gold Rapid Detection Card

Instruction Manual

* The sample pretreatment method of the four metabolites of nitrofuran is exactly the same, and one sample treatment can detect four products at the same time.

1 principle and use

This product is made of the principle of competitive inhibition colloidal gold immunochromatography, which is used to detect furaltadone metabolite residues in tissue samples (chicken, duck, fish, shrimp). The whole detection process takes 2 hours and is suitable for all kinds of enterprises and testing institutions.

2 Technical indicators

Product detection limit: 0.5 μg/kg (ppb)

3 kit composition

test card (containing gold standard micropores, dropper, desiccant) 10;

1 M extractant 1 1 bottle; 1 M sodium hydroxide 1 bottle;

derivative reagent 1 bottle; 0.1 M dipotassium hydrogen phosphate 1 bottle;

reagent A 1 bottle; reagent B 1 bottle;

nitrofuran special complex solution 1 bottle; manual 1 part

4 Need to bring your own equipment and reagents

4 .1 Instruments and consumables: homogenizer, nitrogen drying device/sample concentrator, oscillator, centrifuge, balance (sensitivity 0.01g), water bath, 5 ml centrifuge tube

4 .2 Pipette Single channel 20 µL-200 µL, 100 µL-1000 µL

5 sample pretreatment

5 Take a certain amount of chopped adipose tissue samples and homogenize with a homogenizer of

;

5 Weigh about 2 grams, homogenize in a 50 mL centrifuge tube;

5 Add 4 mL of purified water, 0.5 mL 1M extractant 1, 0.2 mL of derivative reagent in turn, mix well for 3 minutes;

5 Incubate at 60 ° C for 1 hour;

5.5 Remove and add 5 mL 0.1M dipotassium hydrogen phosphate, 0.4

mL 1 M sodium hydroxide, 6 mL reagent A, mix well 3

minutes, centrifuge at 4000 rpm at room temperature (20-25 ° C)

5 minutes;

5.6 with a pipette Pipette 3 ml of the upper layer solution in a 5 mL tube from

, blow dry with nitrogen (empty) gas at 60 ° C, and obtain solid residue

residue;

5 Add 0.5 mL of reagent B to the blow-dried centrifuge tube,

cover and shake for 1 minute; then add 0.5 mL of complex

solution, mix well, and centrifuge at 4000 rpm for 1 minute (or

to stand until obviously stratified);

5 absorb 100 µL of the lower layer solution, pending inspection.

6 sample detection

6 Tear off the aluminum foil bag, take out the detection card, and place it horizontally on the desktop;

6.2 Absorb the above solution to be tested 100 µL, add it vertically to the gold standard micropores, use a dropper or pipette tip to fully mix the red substance in the micropores and the solution to be tested, and let it stand for 5 minutes;

6 Absorb all the liquid in the micropores and add it dropwise to the sample well of the detection card;

6 Start timing after adding the sample, the result should be judged in 8-10 minutes, and the interpretation at other times is invalid. 7 Results Judgment

Negative: The control line (C) appears purplish-red line, and the detection line (T) is darker or as deep as the C line, indicating that the concentration of furanone metabolites in the sample is lower than the detection limit or does not contain furanitone metabolites.

Positive: Purple-red line appears on the control line (C), and the detection line (T) does not show color or is significantly lighter than the C line, indicating that the concentration of furaltadone metabolites in the sample is higher than the detection limit.

Failure: In the detection window, the control line (C) does not appear purplish-red line.

8 Precautions

8 Products that have expired or damaged aluminum foil bags should not be used.

8 When the test card is taken out of the refrigerator, it should be restored to room temperature and opened. The opened test card should be used as soon as possible to avoid failure after moisture.

8 Do not touch the white film surface in the center of the test card.

8.4 The liquid extraction dropper cannot be mixed to avoid cross contamination.

8 The sample solution to be tested should be clear, no cloudy particles, no bacterial contamination, otherwise it will easily lead to blockage, inconspicuous color development and other abnormal phenomena, thus affecting the judgment of experimental results.

9 Safety Description

Ethyl acetate and n-hexane are flammable reagents, and should be used away from ignition sources. If the skin is in contact, rinse thoroughly with soap and water. If the eyes are in contact, the eyelids should be lifted, rinsed with water or normal saline, and seek medical attention. If accidentally ingested, drink enough warm water, induce vomiting, and seek medical attention.

10 Storage and shelf life

10 Storage conditions: 4-30 ° C Store in the dark, do not freeze.

10.2 Shelf life: valid period 1 year, see the box for the production date.