Beta-lactam drug in poultry eggs Rapid detection card

Instruction manual

Product number: YPAW-95

1 principle and use

Beta-lactam drug detection card is used for rapid detection of beta-lactam drug residues in poultry egg samples. This product is made using the principle of competitive inhibition colloidal gold immunochromatography, which is used to detect beta-lactam drugs in samples. After the sample solution is dropped into the sample well of the detection card, the beta-lactam drugs in the sample solution are combined with the gold standard antibody, thereby preventing the gold standard antibody from binding to the beta-lactam drug conjugate on the cellulose membrane. The result is judged according to the color development depth of the T-line and the C-line.

2 Technical Indicators

Detection Drug

Judgment Limit

Detection Drug

Judgment Limit

Amoxicillin

4 μg/kg

Naphthicillin

35μg/kg

Penicillin G

4 μg/kg

Ceftiofur

250μg/kg

Ampicillin

10μg/kg

Cefoperazone

10μg/kg

10μg/kg

Cefquinoxime

30μg/kg

Benzoxicillin 117277981

4 sample pretreatment

(1) Break the fresh eggs into the cup and mix well (egg white and egg yolk are well mixed);

(2) Absorb 0.2 grams of the well-mixed egg sample in the test tube, and then add 1.2 mL dilution;

(3) Shake violently for 1 minute, which is the liquid to be tested;

5 sample detection

(1) Tear open the aluminum foil bag of the test card, take out the test card/gold standard microporous/dropper (a total of 3 kinds of consumables), and place it on a flat and clean countertop.

(2) with a pipette or a dropper in an aluminum foil bag to absorb 120 µL (about 4 drops) of the liquid to be tested in the gold standard micropores, blow with a dropper until the purplish-red substance in the pores is completely dissolved, and let stand horizontally, waiting for the reaction for 2 minutes; all the liquid in the gold standard micropores is added dropwise to the sample well (S) of the detection card;

(3) start timing after adding the sample, and the result is judged after 8-10 minutes at room temperature. The result interpretation for more than 10 minutes is invalid.

6 result judgment

Negative (-): T-line color development is deeper than C-line or there is no significant difference in color development with C-line, indicating that there is no substance to be tested in the sample or its concentration is lower than the detection limit.

Positive (+): The color development of the T-line is significantly weaker than that of the C-line or the T-line does not develop color, indicating that the concentration of the substance to be tested in the sample is equal to or higher than the detection limit.

Invalid: No C-line appears, indicating that the operation process is incorrect or the test card has failed. In this case, the instructions should be read carefully again and re-tested with a new test card.

7 Precautions

(1) Products that have expired or the aluminum foil bag is damaged should not be used.

(2) The test card should be opened after returning to room temperature. The opened test card should be used as soon as possible to avoid failure.

(3) Do not touch the white film surface in the center of the test card.

(4) The liquid extraction dropper cannot be mixed to avoid cross

(5) The liquid to be tested should be clear, free of cloudy particles, and free of bacterial contamination, otherwise it will easily lead to abnormal phenomena such as blockage and inconspicuous color development, which will affect the judgment of experimental results.

(6) Please store the reagent in a place that is not easy for children to contact, and do not eat the equipped reagent.

(7) If you need to directly test the standard product, please prepare it with the diluent provided in the kit.

8 Storage and shelf life

(1) Storage conditions: 4-30 ° C Store in the dark, do not freeze.

(2) Shelf life: 1 year, see the outer packaging for the production date.

9 kit composition

specification

composition

1 times/box

10 times/box

20 times/box

detection card (containing gold standard micropores, droppers, desiccant)

1 parts

10 parts

20 parts

dilution

1 bottle

1 bottle

1 bottle

2 bottle

1 mL detection tube

1 pieces

10 pieces

20 pieces

manual

1 parts

1 parts

1 parts