Triazophos Colloidal Gold Rapid Detection Card Instruction Manual

1 Principle and Use

This product is made of the principle of competitive inhibition of colloidal gold immunochromatography for the detection of triazophos residues. After the sample solution is dropped into the sample hole of the detection card, the triazophos in the sample solution is combined with the gold standard antibody, thereby preventing the gold standard antibody from binding to the triazophos conjugate on the cellulose membrane. The detection result is judged by the color depth of the C and T lines.

2 Technical Indicators

Detection Sensitivity: 0.05 mg/kg

3 You need to bring your own tools

Electronic balance (0.1-100 grams), scissors, disposable cups, forceps, vortex mixer, pipette (0.1-1 mL), timer.

4 sample pretreatment

4 Take the sample epidermis, cut into 3-5 mm width, do not mash;

4 Weigh 2 grams of chopped sample in the sample cup with a balance;

4 Add 2 mL of triazophos dilution with a straw, fully shaken for 10 seconds, after soaking for 5 minutes, as the sample solution; 117277984IX.4 Take a 1.5 mL centrifuge tube, dilute the sample solution with different proportions in the following table, the diluted solution is the liquid to be tested;

Different sample dilution ratios and detection limits

Sample name

Dilution ratio

(sample solution: dilution)

National standard limited GB2763-2021

Leafy vegetables, nightshade vegetables, bean vegetables, aquatic vegetables, sprouts, root vegetables

No dilution,

Direct detection 117277984000.05 mg/kg

Lychee, apple, orange, orange

100µL: 300µL

(1:3)

0 mg/kg

Rice

20µL: 240µL

(1:12)

0 mg/kg

5 Sample testing

5.1 Tear the test card aluminum foil packaging bag, remove the test card/gold microporous/dropper (3 kinds of consumables in total), and place it on a flat and clean countertop.

5 with pipette or aluminum foil bag matching dropper to absorb the liquid to be tested 120 µL (about 4 drops) into the gold standard micropores, the red substance in the micropores and the solution to be tested are fully mixed with the dropper, let stand for 2 minutes, and then mix;

5 All the liquid in the gold standard micropores is added dropwise to the sample well (S) of the detection card;

5 Start timing after adding the sample, and leave it at room temperature for 8-10 minutes to judge the result, and the interpretation is invalid at other times.

6 result judgment

negative (-): T-line color development is deeper than C-line or there is no significant difference in color development with C-line, indicating that there is no substance to be tested in the sample or its concentration is lower than the detection limit.

Positive (+): The color development of the T-line is significantly weaker than that of the C-line or the T-line does not develop color, indicating that the concentration of the substance to be tested in the sample is equal to or higher than the detection limit.

Invalid: The C-line does not appear, indicating that the operation process is incorrect or the test card has failed. In this case, the instructions should be read carefully again and re-tested with a new test card.

7 Precautions

7 Products that have expired or the aluminum foil bag is damaged should not be used.

7 When the test card is taken out of the refrigerator, it should be restored to room temperature and opened. The opened test card should be used as soon as possible to avoid failure after being damp.

7 Do not touch the white film surface in the center of the test card.

7.4 The liquid extraction dropper should not be mixed to avoid cross

7 The sample solution to be tested should be clear, no cloudy particles, and no bacterial contamination, otherwise it will easily lead to abnormal phenomena such as blockage and inconspicuous color development, which will affect the judgment of experimental results.

7 If you need to directly test the standard product, you need to use the triazophos dilution in the kit.

8 Storage and shelf life

8 Storage conditions: 4-30 ℃ Store in the dark, do not freeze.

8.2 Shelf life: valid period 1 year, see the outer packaging for the production date.

9 kit composition

specification

composition

10 times/box

20 times/box

test card (containing gold standard micropores, dropper, desiccant)

10 parts

20 parts

triazophos dilution

1 bottle

2

15 mL centrifuge tube

2

4

4

1 mL centrifuge tube

10

20

manual

1

1 parts