Amithiophosphorus hydrocolloidal gold rapid detection card instruction manual
1 principle and use
This product applies the principle of competition inhibition colloidal gold immunochromatography for the detection of water amthiophosphorus residues in vegetables and fruits. After the sample solution is dropped into the sample hole of the detection card, the water amthiophosphorus in the sample solution is combined with the gold standard antibody to prevent the gold standard antibody from binding to the water amthiophosphorus conjugate on the cellulose membrane. The detection result is judged by the color depth of C and T lines.
2 technical indicators, the minimum detection limit: 0.05 mg/kg (ppm)
3 You need to bring your own tools
electronic balance (0.1-100 grams), scissors, forceps, 0.1-1 mL pipette (optional), timer.
4 sample pretreatment
4 The sample needs to be restored to room temperature (20-30 ° C) before testing.
4.2 Take an appropriate amount of sample, cut it into 1 cm square fragments, weigh 1 gram of the shredded sample in a 20 mL scale sample cup, add 4 mL dilution, manually shake the extraction for 2 minutes, and let stand for 1 minute to obtain the sample to be tested;
* If the more viscous samples such as strawberries are detected, the test solution may affect the test results, and the dilution ratio can be increased, for example, 2 grams of sample + 5 mL dilution, the extracted solution is used as the test solution.
5 sample detection
(1) Tear open the aluminum foil packaging bag of the detection card, take out the detection card/gold standard micropore/dropper (a total of 3 kinds of consumables), and put it on a flat and clean countertop.
(2) Use a pipette or an aluminum foil bag with a matching dropper to absorb the above 120µL (about 4 drops) of the liquid to be tested in the gold standard micropore, blow with the dropper until the purplish-red substance in the hole is completely dissolved, let it stand horizontally, and wait for the reaction for 2 minutes; add all the liquid in the gold standard micropore to the sample hole (S) of the detection card;
(3) Start timing after adding the sample, and leave it at room temperature for 8-10 minutes to judge the result. The result interpretation at other times is invalid.
6 result judgment
Negative (-): The color development of the T-line is darker than that of the C-line or there is no significant difference from that of the C-line, indicating that there is no substance to be tested in the sample or its concentration is below the detection limit.
Positive (+): The color development of the T-line is significantly weaker than that of the C-line or the T-line does not develop color, indicating that the concentration of the substance to be tested in the sample is equal to or higher than the detection limit.
Invalid: No C-line appears, indicating that the operation process is incorrect or the test card has failed. In this case, the instructions should be read carefully again and re-tested with a new test card.
7 Precautions
7.1 Products that have expired or the aluminum foil bag
7 When the test card is taken out from the refrigerator, it should be restored to room temperature and opened. The opened test card should be used as soon as possible to avoid failure after dampness.
7 Do not touch the white film surface in the center of the test card.
7 The liquid dropper should not be mixed to avoid cross-contamination.
7. 5 The sample solution to be tested should be clear, no cloudy particles, no bacterial contamination, otherwise it will easily lead to abnormal color development and other phenomena, which will affect the result judgment.
8 Storage and shelf life
8 Storage conditions: 4-30 ℃ in the dark, do not freeze.
8.2 Shelf life: valid period 1 year, see the outer packaging for the production date.
9 kit composition
specification
composition
10 times/box
20 times/box
test card (containing gold standard micropores, dropper, desiccant)
10 parts
20 parts
water aminophosphorus dilution
1 bottle
2 bottle
20 mL graduated sample cup
(can be recycled after cleaning)
1
2
1 mL straw
10
20
manual
1
1 parts
1 parts