Oxytetracycline Colloidal Gold Rapid Detection Card

Instruction Manual

This product is made by the principle of competitive inhibition colloidal gold immunochromatography for detecting oxytetracycline in samples. After the sample solution is dropped into the sample well of the test card, the oxytetracycline in the sample solution binds to the gold standard antibody, thereby preventing the gold standard antibody from binding to the oxytetracycline conjugate on the cellulose membrane. The result is judged according to the color development depth of the T-line and the C-line.

2 technical index

Detection limit: 100μg/kg (ppb)

3 need to bring your own tools

Homogenizer/cooking machine, vortex mixer (optional), centrifuge, electronic balance, pipette (0.1-1 mL), timer.

4 sample pretreatment

(1) Take a certain amount of chopped adipose tissue samples, homogenized with a homogenizer;

(2) Weigh about 1 gram of homogeneous material in a 5mL detection tube;

(3) Add 1 mL of reagent A and shake violently for 5 minutes;

(4) centrifuge at 4000 rpm for 5 minutes at room temperature;

(5) absorb 0.5 mL of supernatant in a 1.5mL centrifuge tube, add 80 μL of reagent B, shake and mix well, centrifuge at 4000 rpm for 2 minutes at room temperature;

(6) Supernatant is used as the liquid to be tested for standby;

5 sample detection

(1) Tear off the aluminum foil bag of the detection card, take out the detection card/gold standard microporous/dropper (a total of 3 kinds of consumables), and place in On a flat, clean countertop.

(2) with a pipette or a dropper in an aluminum foil bag to absorb the above test solution 120 µL (about 4 drops) in the gold standard micropores, blow with a dropper until the purplish-red substance in the pores is completely dissolved, let stand horizontally, and wait for the reaction for 2 minutes; all the liquid in the gold standard micropores is added dropwise to the sample well (S) of the detection card;

(3) Start timing after adding the sample, and the result is judged after 8-10 minutes at room temperature, and the interpretation is invalid at other times.

6 the result is judged

negative (-): the purplish-red line appears on the control line (C), and the color development of the detection line (T) is consistent with or deeper than that of the C line, indicating that the concentration of oxytetracycline in the sample is lower than the detection limit

Positive (+): Purple red line appears on line C, T line does not develop color or is lighter than line C, indicating that the concentration of oxytetracycline in the sample is higher than the detection limit.

Invalid: Control line (C) does not appear purplish red line.

7 Precautions

(1) Products that have expired or the aluminum foil bag is damaged should not be used.

(2) When the test card is taken out of the refrigerator, it should be restored to room temperature and opened. The opened test card should be used as soon as possible to avoid failure after moisture.

(3) Do not touch the white film surface in the center of the test card.

(4) The liquid extraction dropper cannot be mixed to avoid cross-contamination.

(5) The liquid to be tested should be clear, free of cloudy particles, and free of bacterial contamination, otherwise it will easily lead to abnormal phenomena such as blockage and inconspicuous color development, which will affect the judgment of experimental results.

(6) Please store the reagent in a place that is not easy for children to contact, and do not eat the equipped reagent.

(7) If you need to directly test the standard product, please prepare it with the diluent provided in the kit.

8 Storage and shelf life

(1) Storage conditions: 4-30 ° C Store in the dark, do not freeze.

(2) Shelf life: 1 year, see the outer packaging for the production date.

9 kit composition

specification

composition

1 times/box

10 times/box

20 times/box

detection card (containing gold standard micropores, droppers, desiccant)

1 parts

10 parts

20 parts

oxytetracycline dilution

1 bottle

1 bottle

1 bottle

2 bottle

5ml frozen tube

1 pieces

10 pieces

20 pieces

172779840011 5 ml centrifuge tube

1 pieces

10 pieces

20 pieces

manual

1 parts

1 parts

1 parts 1