Tissue Chlortetracycline Colloidal Gold Rapid Detection Card Instruction Manual

Product Number: YB128R01K

Summary

Chlortetracycline (chlortetracycline) is a highly effective broad-spectrum antibiotic, which has strong inhibitory effect on a variety of pathogenic bacteria, and is often used in the treatment of various infectious diseases in animals. However, its residues in animal meat, milk, eggs and other foods pose a serious threat to human health, and can cause diseases such as aplastic anemia and granulocytic leukemia. Low concentrations of drug residues can also induce drug resistance of pathogenic bacteria. Many countries such as the European Union and the United States have clearly banned the use of CAP in the production of animal-derived foods, and have formulated strict limit standards.

Detection principle

This product is made using the principle of competitive inhibition colloidal gold immunochromatography to detect chlortetracycline residues in tissue samples. After the sample solution is dropped into the sample hole of the test card, the sulfonamide drug in the sample solution is combined with the gold standard antibody, thereby preventing the gold standard antibody from binding to the sulfonamide drug conjugate on the cellulose membrane. According to the color development depth of the T-line and the C-line judgment results.

Scope of application

This product is suitable for qualitative detection of chlortetracycline residues in aquatic products, livestock and poultry tissue samples.

Note: For the type of test sample, refer to the national standard GB2763-2021.

detection limit

chlortetracycline detection limit: 200μg/kg (ppb)

Kit composition

serial number

Specification

Composition

10 times/box

20 times/box

(1)

detection card (containing gold standard micropores, droppers, desiccant)

10 parts

20 parts

(2)

chlortetracycline dilution

1 bottle

2 bottle

(3)

5ml centrifuge tube

10 pieces

20 pieces

(4)

Instructions

1 parts

1 parts

Precautions

(1) Before each sample, the knife for shredding the sample needs to be cleaned to avoid cross-contamination.

(2

(3) Please follow the test steps for testing. Do not touch the color-developing area of the test strip during operation to avoid direct sunlight and direct blowing of electric fans.

(4) Please use the sample as soon as possible after processing. If the time is too long, you need to re-process the sample for re-testing.

(5) The solution of the sample to be tested needs to be clarified, otherwise it will easily lead to abnormal phenomena such as inconspicuous color development, which will affect the judgment of the experimental results.

(6) Products that have expired or damaged aluminum foil bags cannot be used. Please use the test card immediately after unpacking.

(7) This product is a one-time product. Do not reuse or mix test cards from different batches.

(8) When a positive result appears, it is recommended to re-test. The test results of this product are for reference only. If you need confirmation, please refer to the relevant national standards and methods.

(9) During product evaluation, if the standard product needs to be tested directly, it needs to be prepared with the special dilution solution in the kit.

(10) Tap water, distilled water, purified water or deionized water cannot be used as a negative control.

Safety instructions

(1) The experiment needs to match the corresponding experimental equipment and wear the necessary experimental equipment (white clothes, gloves, masks, etc.).

(2) The detection kit needs to be stored in a place that is not easy for children to contact.

(3) All items used in the experiment should be properly disposed of after application.

(4) Keep the laboratory clean and the air flow of the experimental environment after the experiment.

(5) The experimental waste should be collected separately, and it is recommended to dispose of it as medical waste.

(6) Do not eat the equipped reagents.

storage conditions and valid period

(1) Storage conditions: 2-30 ℃ protected from light, do not freeze.

(2) valid period: 12 months.