Furacillin Metabolite Colloidal Gold Rapid Detection Card

Instruction Manual

* The sample pretreatment methods of the four nitrofuran metabolites are exactly the same, and one sample treatment can detect four products at the same time.

1 Principle and Use

This product is made of the principle of competitive inhibition colloidal gold immunochromatography, which is used to detect furacillin metabolite residues in tissue samples (chicken, duck, fish, shrimp). The whole detection process takes 2 hours and is suitable for all kinds of enterprises and testing institutions.

2 Technical indicators

Product detection limit: 0.5 μg/kg (ppb)

3 kit composition

test card (containing gold standard micropores, dropper, desiccant) 10;

1 M extractant 1 1 bottle; 1 M sodium hydroxide 1 bottle;

derivative reagent 1 bottle; 0.1 M dipotassium hydrogen phosphate 1 bottle;

reagent A 1 bottle; reagent B 1 bottle;

nitrofuran special complex solution 1 bottle; manual 1 part

4 Need to bring your own equipment and reagents

4 .1 Instruments and consumables: homogenizer, nitrogen drying device/sample concentrator, oscillator, centrifuge, balance (sensitivity 0.01g), water bath, 5 ml centrifuge tube

4 .2 Pipette Single channel 20 µL-200 µL, 100 µL-1000 µL

5 sample pretreatment

5 Take a certain amount of chopped adipose tissue samples and homogenize with a homogenizer of

;

5 Weigh about 2 grams, homogenize in a 50 mL centrifuge tube;

5 Add 4 mL of purified water, 0.5 mL 1M extractant 1, 0.2 mL of derivative reagent, fully shake and mix for 3 minutes;

5 Incubate in a water bath at 60 ° C for 1 hour;

5.5 Remove and add 5 mL 0.1M dipotassium hydrogen phosphate, 0.4

mL 1 M sodium hydroxide, 6 mL reagent A, fully mix 3

minutes, centrifuge at 4000 rpm at room temperature (20-25 ° C)

5 minutes;

5.6 with transfer Liquid suction 3 ml of the upper layer solution in 5 mL from

heart tube, 60 ℃ nitrogen (empty) air drying, to give solid residue

slag;

5 was added to the blow-dried centrifuge tube 0.5 mL of reagent B,

cover oscillation mixing for 1 minute; then add 0.5 mL complex

solution, thoroughly mixed, 4000 rpm centrifuged for 1 minute (or

to stand until significantly stratified);

5 the lower layer solution 100 µL, standby.

6 sample detection

6 Tear off the aluminum foil bag, take out the detection card, and place it horizontally on the desktop;

6 Absorb the above solution to be tested 100 µL, add it vertically to the gold standard micropores, use a dropper or pipette tip to fully mix the red substance in the micropores and the solution to be tested, and let it stand for 5 minutes;

6.3 Absorb all the liquid in the micropores and add it to the sample well of the detection card;

6.4 Start timing after adding the sample, the result should be judged in 8-10 minutes, and the interpretation at other times is invalid. 7 The result is

negative: the control line (C) appears purplish-red line, and the color development of the detection line (T) is deeper or the same as that of the C line, indicating that the concentration of furacillin metabolites in the sample is lower than the detection limit or does not contain furacillin metabolites.

Positive: Purple-red line appears on the control line (C), and the detection line (T) does not show color or is significantly lighter than the C line, indicating that the concentration of furacillin metabolites in the sample is higher than the detection limit.

Failure: In the detection window, the control line (C) does not appear purplish-red line.

8 Precautions

8 Products that have expired or damaged aluminum foil bags should not be used.

8 When the test card is taken out of the refrigerator, it should be restored to room temperature and opened. The opened test card should be used as soon as possible to avoid failure after moisture.

8 Do not touch the white film surface in the center of the test card.

8.4 The liquid extraction dropper cannot be mixed to avoid cross contamination.

8. 5 The sample solution to be tested should be clear, free of cloudy particles, and free of bacterial contamination, otherwise it will easily lead to abnormal phenomena such as blockage and inconspicuous color development, which will affect the judgment of experimental results.

9 Safety Description

Ethyl acetate and n-hexane are flammable reagents, and keep away from ignition sources when using. If the skin is in contact, rinse thoroughly with soap and water. If the eyes are in contact, lift the eyelids, rinse with water or normal saline, and seek medical attention. If you accidentally eat it, drink enough warm water, induce vomiting, and seek medical attention.

10 Storage and shelf life

10 Storage conditions: 4-30 ° C Store in the dark, do not freeze.

10.2 Shelf life: valid period 1 year, see the box for the production date.