Dichloroquinolinic acid colloidal gold rapid detection card

Instruction manual

1 principle and use

This product is made of the principle of competitive inhibition colloidal gold immunochromatography for the detection of dichloroquinolinic acid pesticide residues in vegetables and fruits. After the sample solution is dropped into the sample hole of the detection card, the dichloroquinolinic acid in the sample solution binds to the gold standard antibody, thereby preventing the gold standard antibody from binding to the dichloroquinolinic acid conjugate on the cellulose membrane. The detection result is judged by the color depth of the C and T lines.

2 Technical indicators

Detection sensitivity: 200 μg/kg (ppb)

3 kit consists of

test card (containing dropper, desiccant) 10;

dichloroquinolinic acid dilution 1 bottle;

sample cup 10; manual 1

4 You need to bring your own tools

electronic balance (0.1-100 g), scissors, forceps, pipette (20 µL-200 µL, 100 µL-1000 µL), timer.

5 sample pretreatment

to take an appropriate amount of sample, cut into a length of 2-3 mm, weighed 1 gram of the cut sample to be tested in the sample cup, and then add 1 ml of quinolinic acid, fully shaken for 2 minutes, to be tested;

6 sample detection

6 Tear off the aluminum foil bag, remove the detection card and place it horizontally on the table;

6 with a dropper to absorb the sample solution to be tested, add 4 drops of

(120 µL) vertically to the detection card in the sample hole (S);

6.3 start timing after adding the sample, and leave it at room temperature for 8-10 minutes to judge the result, other results are invalid.

7 result judgment

Negative (-): The color development of the T-line is darker than that of the C-line or there is no significant difference from that of the C-line, indicating that there is no substance to be tested in the sample or its concentration is below the detection limit.

Positive (+): The color development of the T-line is significantly weaker than that of the C-line or the T-line does not develop color, indicating that the concentration of the substance to be tested in the sample is equal to or higher than the detection limit.

Invalid: No C-line appears, indicating that the operation process is incorrect or the test card has failed. In this case, the instructions should be read carefully again and re-tested with a new test card.

8 Precautions

8.1 Products that have expired or the aluminum foil bag is damaged should not be used.

8 When the test card is taken out of the refrigerator, it should be restored to room temperature and opened. The opened test card should be used as soon as possible to avoid failure after dampness.

8 Do not touch the white film surface in the center of the test card.

8 The liquid dropper should not be mixed to avoid cross-contamination.

8 The sample solution to be tested should be clear, free of turbid particles, and free of bacterial contamination, otherwise it will easily lead to abnormal phenomena such as blockage and insignificant color development, thus affecting the judgment of the experimental results.

8 If you need to directly test the standard product, you need to prepare the dilute of dichloroquinolinic acid in the kit.

9 Storage and shelf life

9 Storage conditions: 4-30 ° C Store in the dark, do not freeze.

9.2 Shelf life: valid period 1 year, see the outer packaging for the production date.