Pendimethalin Colloidal Gold Rapid Detection Card Instruction Manual

1 Principle and Use

This product is made of the principle of competitive inhibition of colloidal gold immunochromatography for the detection of pendimethalin pesticide residues in vegetables and fruits. After the sample solution is dropped into the sample hole of the detection card, the pendimethalin in the sample solution binds to the gold standard antibody, thereby preventing the gold standard antibody from binding to the dimethalin conjugate on the cellulose membrane. The detection result is judged by the color depth of the C and T lines.

2 Technical indicators

Detection sensitivity: 5 mg/kg (ppm)

3 kit composed of

test card (containing dropper, desiccant) 10;

dimethylpentyl dilution 1 bottle; sample cup 10

Instructions 1 copy

4 need to bring your own equipment and reagents 117277984iron.1 Instrument: balance (sensitivity 0.01g) 117277984iron.2 Micropipette: single channel 20 µL-200 µL, 100 µL-1000 µL

5 Sample pretreatment

Take an appropriate amount of sample, cut it into 2-3 mm length, weigh the shredded sample 1.0 g in the sample cup, add 1 ml dimethylpentyl dilution, fully soak and shake for 2 minutes, pending inspection;

6 sample detection

6 Tear off the aluminum foil bag, take out the test card, and place it horizontally on the table top;

6.2 Absorb the sample solution to be tested with a dropper, and add 4 drops (120 µL) vertically to the sample hole (S) of the test card;

6. 3 Start timing after adding the sample, and leave it at room temperature for 8-10 minutes to judge the result. Other results are invalid.

7 The result is judged

negative: both the control line (C) and the detection line (T) have purplish-red lines, indicating that the concentration of dimethylpendimethyl in the sample is lower than the detection limit or there is no dimethylpendimethyl residue.

Positive: Purple red line appears on the control line (C), and the test line (T) does not show color, indicating that the concentration of dimethylpendimethalin in the sample is higher than the detection limit.

Failure: In the detection window, the control line (C) does not appear purplish red line.

8 Precautions

8 Products that have expired or the aluminum foil bag is damaged cannot be used.

8 When the test card is taken out of the refrigerator, it should be restored to room temperature and opened. The opened test card should be used as soon as possible to avoid failure after dampness.

8 Do not touch the white film surface in the center of the test card.

8 The liquid extraction dropper should not be mixed to avoid cross-contamination.

8.5 The sample solution to be tested should be clear, free of cloudy particles and bacterial contamination, otherwise it will easily lead to abnormal phenomena such as blockage and insignificant color development, which will affect the judgment of experimental results.

8 If you need to directly test the standard product, you need to prepare the dilute solution of dimethalin in the kit.

9 Storage and shelf life

9 Storage conditions: 4-30 ℃ in the dark, do not freeze.

9.2 Shelf life: valid period 1 year, see the outer packaging for the production date.