Sodium pentachlorophenate colloidal gold rapid detection card is a rapid detection product based on colloidal gold immunochromatography technology in the field of food safety testing. It is mainly used for rapid screening of sodium pentachlorophenate residues in food. It completes the detection in a short time through specific immune reaction principles, providing efficient residue monitoring tools for regulatory authorities, food companies and scientific research institutions.
From the technical principle, the detection card takes colloidal gold-labeled sodium pentachlorophenate antibody as the core, and combines the detection line (coated sodium pentachlorophenate antigen) and quality control line (coated antibody) on the nitrocellulose membrane. When the sample is added dropwise to the detection card and the sample well is added, it moves forward under capillary action. If sodium pentachlorophenate is present in the sample, it will bind to the colloidal gold-labeled antibody to form a complex. When it continues to move to the detection line, if the concentration of sodium pentachlorophenate is sufficient, the complex will compete with the detection line antigen to bind the antibody, resulting in the detection line not being colored; conversely, the detection line is colored. The quality control line is used to verify whether the detection process is normal and ensure the reliability of the results.
Structurally, the detection card is usually composed of a sample pad, a binding pad (containing colloidal gold-labeled antibodies), a nitrocellulose membrane (including the detection line and quality control line), an absorbent pad, and a PVC board. Each part is assembled through a
Compared with traditional detection methods, sodium pentachlorophenate colloidal gold rapid detection card has significant advantages: first, the detection speed is fast, the whole process only takes 10-15 minutes, no need for complex instruments; second, the operation is simple, the user only needs to simply pre-process the sample (such as extraction, dilution), according to the instructions after dripping observation results; third, high sensitivity, can detect as low as 0.1-1 ng/mL residual concentration; fourth, strong portability, small size, suitable for on-site detection or laboratory rapid screening.
At present, the detection card is widely used in food safety supervision, especially for meat, aquatic products, vegetables and fruits and other foods in the rapid screening of sodium pentachlorophenate residues, can effectively help the relevant units to detect the problem of excessive standards in time, to ensure consumer food safety.