What is metronidazole colloidal gold rapid detection card?

Metronidazole Colloidal Gold Rapid Detection Card is a rapid screening product based on colloidal gold immunochromatography technology in the field of food safety testing. It is mainly used to detect the residue of metronidazole in animal-derived foods (such as pork, chicken, milk, aquatic products, etc.). It can perform qualitative or semi-quantitative analysis of metronidazole in samples in a short time, providing efficient detection support for food safety supervision, enterprise production quality control and other scenarios.

From the perspective of product composition, the detection card is usually composed of sample pad, binding pad, nitrocellulose membrane, absorbent pad and PVC board. Among them, the binding pad is labeled with colloidal gold-labeled metronidazole monoclonal antibody, and the nitrocellulose membrane is coated with metronidazole antigen (detection line) and sheep anti-mouse IgG antibody (quality control line). When the sample (such as tissue homogenate, liquid sample, etc.) is added dropwise to the sample well, it will move on the test strip through capillary action, and flow through the binding pad, nitrocellulose membrane and absorbent pad in turn.

In terms of detection principle, if the sample contains metronidazole, it will bind to the colloidal gold-labeled antibody on the binding pad to form an antigen-antibody complex. When the composite logistics passes through the detection line of nitrocellulose membrane, due to the competitive relationship between the antigen and the antigen coated with the detection line, the composite will be bound by the antigen of the detection line, resulting in no color rendering of the detection line; conversely, if there is no metronidazole in the sample, the colloidal gold-labeled antibody will bind to the antigen of the detection line, so that the detection line will develop color. At the same time, the quality control line will always develop color, which serves as an effective control for the reaction.

Compared with traditional detection methods, the metronidazole colloidal gold rapid detection card has significant advantages: the detection speed is fast, and the results can be produced in 10-15 minutes usually; the operation is simple, without the need for professional equipment and complex pretreatment; the sensitivity is high, which can meet the detection needs of the residue limit standard Therefore, it plays an important role in the quality and safety control of animal source food production, processing, circulation and other links, which can help relevant departments quickly identify substandard products and ensure consumer dietary safety.