As a broad-spectrum antimicrobial veterinary drug, florfenicol is widely used in aquaculture and livestock and poultry breeding for the treatment of bacterial infectious diseases. However, if used improperly or added in excess, it may lead to its residue in animals, and after entering the human body through the food chain, it will pose a potential threat to health, such as causing blood system abnormalities, allergic reactions, etc. Therefore, the rapid and accurate detection of florfenicol residues in aquaculture products is essential, and the florfenicol colloidal gold rapid detection card is a powerful tool to meet this demand.
florfenicol colloidal gold rapid detection card is a rapid detection test strip developed by colloidal gold immunochromatography technology. The core principle is the use of antigen-antibody specific binding reaction: the detection line (T-line) of the detection card is coated with florfenicol antigen, and the quality control line (C-line) is coated with sheep anti-mouse IgG antibody; when the sample (such as tissue extract, milk, urine, etc.) is added dropwise to the sample well, it will move along the chromatography strip. If the sample contains florfenicol, it will combine with the colloidal gold-labeled florfenicol antibody to form a complex. When moving to the detection line, the complex competes with the T-line antigen to bind the colloidal gold-labeled antibody. If the florfenicol concentration in the sample is lower than the threshold, the complex will bind to the T-line antigen to make the T-line color develop; the C-line is used to determine whether Whether the sample contains fluorfenicol or not, the C-line will develop color, thus enabling quick judgment results.
This detection card has significant advantages: first, the detection speed is fast, usually 10-15 minutes to produce results, which greatly shortens the time-consuming of traditional detection methods (such as high-performance liquid chromatography, mass spectrometry, etc.); second, it is convenient to operate, without the need for professional instruments and complex operation steps. It only needs to simply extract the sample, add it to the detection card, and observe the color development with the naked eye. Third, it has high sensitivity, which can reach the ng/mL level, and can accurately detect low-concentration residues. Fourth, it is low-cost and suitable for batch use in grass-roots testing institutions, farms, slaughterhouses, and other scenarios.
In practical applications, the florfenicol colloidal gold rapid detection card is mainly used for prenatal, intrapartum and postpartum monitoring in the breeding process, such as rapid screening of tissue and body fluid samples of fish, pigs, chickens and other cultured products, and can also be used for market supervision departments to sample products on the market. Through rapid detection, the illegal use of florfenicol can be detected in time, effectively reducing food safety risks and protecting consumer health.
In short, florfenicol colloidal gold rapid detection card has become an important tool in the field of food safety testing with its fast, convenient, sensitive and economical characteristics, providing strong support for guarding the "safety on the tip of the tongue".