Clenbuterol hydrochloride, also known as "clenbuterol", is a substance that is extremely harmful to human health. After it is illegally added to the feed, it will leave a large amount of residue in the animal body. Eating meat containing such substances may cause symptoms such as heart palpitations and muscle tremors, and even life-threatening in severe cases. Therefore, the rapid and accurate detection of clenbuterol hydrochloride in food is an important link to ensure food safety. The Clenbuterol Hydrochloride Colloidal Gold Rapid Detection Card is a practical detection tool that came into being in this context.
By definition, the Clenbuterol Hydrochloride Colloidal Gold Rapid Detection Card is a rapid detection test strip based on colloidal gold immunochromatography technology. It is mainly composed of sample pad, binding pad, nitrocellulose membrane (detection line and quality control line) and absorbent paper. Among them, the clenbuterol hydrochloride-bovine serum albumin conjugate labeled with colloidal gold is fixed on the binding pad, the detection line on the nitrocellulose membrane is coated with clenbuterol hydrochloride monoclonal antibody, and the quality control line is coated with sheep anti-mouse IgG antibody.
Its working principle is not complicated: when the sample to be tested (such as pork homogenate, pig liver extract, etc.) is added dropwise to the loading hole of the detection card, the sample will move forward along the test strip and flow through the binding pad and nitrocellulose membrane in turn. In the binding pad, clenbuterol in the sample will compete with the colloidal gold-labeled antibody to bind the conjugate on the binding pad. If the sample contains clenbuterol hydrochloride and the concentration reaches the detection limit, it will bind to part of the colloidal gold-labeled antibody and prevent this part of the antibody from continuing to move to the detection line. At this time, the detection line will appear colorless or light pink; conversely, if the sample does not contain clenbuterol hydrochloride or the concentration is below the detection limit, the colloidal gold-labeled antibody will bind to the monoclonal antibody of the detection line to cause the detection line to develop color. Whether the quality control line is colored or not, as long as the test strip is operated correctly, it will develop color and be used to determine whether the detection process is effective.
As a rapid detection tool, Clenbuterol Hydrochloride Colloidal Gold Rapid Detection Card has significant advantages. First of all, it is fast. The whole detection process usually only takes 10-15 minutes, and it can quickly produce results, which is suitable for on-site screening. Secondly, it is easy to operate. It does not require complicated instruments and professional training. Ordinary personnel can complete the detection according to the instructions. Furthermore, it has high sensitivity and can detect the residue of ng/mL level, which can accurately identify the low concentration of clenbuterol pollution. In addition, it has low cost and is suitable for large-scale and normalized detection needs.
In the application scenario, it is widely used in the raw material acceptance, processing process monitoring of food production enterprises, origin quarantine and market supervision of agricultural and rural departments, as well as the daily screening of third-party testing agencies. By using the test card, it is possible to detect the residual problem of clenbuterol in food in time, effectively prevent food safety risks, and protect consumers' table safety.
In short, Clenbuterol hydrochloride colloidal gold rapid test card with its fast, sensitive and simple characteristics has become the right-hand assistant for screening clenbuterol in the field of food safety testing, and plays an important role in building a safe food environment.