Thiamethione Colloidal Gold Rapid Detection Card in Mango

Mango Thiamethione Colloidal Gold Rapid Detection Card Instruction Manual

Product number
: YB083C01K

I. summary

Thiamethione is a neonicotinoid insecticide that mainly acts on the nervous system of insects. It can selectively bind to nicotinic acetylcholine receptors in insects, block the transmission of nerve signals, and finally kill pests. It has the characteristics of high efficiency, strong internal absorption, long shelf life and high safety, and is widely used in pest prevention and control of mango planting. As a mainstream fresh fruit, mango is prone to the risk of thiamethione residues in planting, harvesting and circulation. Excessive residues will endanger human health. This product can quickly screen mango samples for thiamethione residues, and is suitable for rapid detection scenarios of various fruit and vegetable food safety.

Second, the detection principle

This product adopts the principle of competitive inhibition immunochromatography. The specific antibody labeled with colloidal gold binds to thiamethione in the sample, which inhibits the binding of the antibody to the antigen on the NC membrane detection line (T line), resulting in the change of the color depth of the T line; according to the color depth judgment result of the T line and the C line.

III. Purpose

It is used for rapid primary screening and detection of thiamethione content in fresh mango samples, and is suitable for on-site rapid screening scenarios such as fruit and vegetable planting bases, fresh food purchases, farmers markets, food processing plants, and food safety supervision and sampling inspections.

IV. Minimum detection limit for samples

0.01 mg / kg (ppm)

V. Cross-reactivity and product performance

This product has accurate specific identification of thiamethione and strong anti-interference ability. The crossover rate with imidacloprid is about 1.5%, and the crossover rate with furfuran, amidacloprid, thiamethoxam and domicillin is less than 0.5%. The detection results are stable and reliable.

Six, the kit composition

VII. Operational Precautions

1. Tools such as knives, scissors, and stickers used to treat samples before testing should be cleaned to avoid cross-contamination.

2. Sample requirements: Avoid using spoiled and deteriorated samples; clean large pieces of soil and impurities on the surface of the mango to avoid dirt interfering with the test results.

3. Before testing, it is recommended that the sample be fully stirred and mixed. If the sampling amount is small, it is necessary to select representative parts such as mango pulp to sample and weigh to ensure that the test results truly reflect the actual drug residue of the sample.

4. The detection environment temperature should be controlled at 17 to 30 degrees Celsius. Excessive or low temperatures can affect the immune chromatography reaction, resulting in deviations in the detection results.

5. Please strictly follow the detection steps for testing. Do not touch the color rendering area of the test strip during operation, and avoid direct sunlight and direct blowing of the electric fan to the detection card.

6. The sample solution to be tested needs to be kept clear, and the turbidity of the solution can easily lead to inconspicuous color development and abnormal base color, which will affect the judgment of the experimental results.

7. Products that have expired or the aluminum foil bag is damaged cannot be used. Please use the test card immediately after unpacking to prevent moisture and failure.

8. Please test the sample as soon as possible after processing. If it takes too long to leave, you need to reprocess the sample before testing.

9. When a positive result appears, it is recommended to retest. The test results of this product are for reference only. If you need accurate confirmation, please refer to the relevant national standard methods for testing.

10. When adding standards for verification, methanol is generally selected as the solvent for the standard solution, and the final sample dosage is recommended to be controlled at 10-50 μL.

11. When directly testing the standard, the solvent should be used with the extract of the kit, and the amount of organic solvent added should be controlled within 1%. Tap water, distilled water, purified water or deionized water cannot be used as negative controls.

VIII. Safety instructions

1. The experiment needs to match the corresponding experimental equipment, and standardize the wearing of white clothes, gloves, masks and other necessary experimental equipment.

2. The test kit should be properly stored and placed in a place that is not easily accessible to children to prevent accidental ingestion.

3. After the experiment, keep the laboratory clean and tidy to ensure air circulation in the experimental environment.

4. This product is a one-time product and cannot be reused. After the test is completed, it needs to be properly disposed of. The experimental waste is collected separately. It is recommended to dispose of it in accordance with the medical waste specification.

5. The reagents involved in this product are safe and reliable, and do not contain carcinogenic, highly toxic, flammable, explosive, and strongly corrosive reagents, but consumption is strictly prohibited.

Storage conditions and valid period

1. Original packaging: 4-30 ℃ in the dark dry storage, do not freeze, valid period of 12 months.

2. After unpacking: Use the test card immediately after unpacking, do not freeze.