Rapid Detection Card for Colloidal Gold in Legume Vegetables

Thiamethione Colloidal Gold Rapid Detection Card in Beans and Vegetables Instruction Manual

Product number
: YB083C01K

I. summary

Thiamethione is a neonicotinoid insecticide, which mainly acts on the nervous system of insects. It can selectively bind to the nicotinic acetylcholine receptor in insects, block the transmission of nerve signals, and finally kill pests. The agent has the characteristics of high efficiency, strong internal absorption, long shelf life and high safety. It is widely used in the cultivation of bean vegetables such as cowpea, kidney beans, lentils, and green beans for insect control. Long-term intake of beans and vegetables with excessive thiamethione residues will endanger human health. This product can realize rapid screening of thiamethione residues in beans and vegetables to ensure the safety of fruit and vegetable consumption.

Second, the detection principle

This product adopts the principle of competitive inhibition immunochromatography. The binding of thiamethione in the sample to the specific antibody labeled with colloidal gold inhibits the binding of the antibody to the antigen on the NC membrane detection line (T line), resulting in changes in the color depth of the T line; according to the color depth of the T line and the C line, the residue of thiamethione in the sample can be judged.

III. Purpose

specifically designed for
Cowpeas, beans, lentils, green beans, peas
On-site rapid screening and detection of thiamethione content in fresh bean and vegetable samples is suitable for various scenarios such as planting bases, farmers' markets, supermarkets, food processing plants, and food safety sampling inspections.

IV. Minimum detection limit for samples

0.01 mg / kg (ppm)

V. Cross-reactivity and product performance

This product has excellent specificity and accurate identification of thiamethione; the crossover rate with imidacloprid is about 1.5%, and the crossover rate with furfuran, amidacloprid, thiamethoxam, and domicillin is less than 0.5%. There is almost no cross interference, and the detection results are stable and reliable.

Six, the kit composition

VII. Operational Precautions

1. Knives, scissors, cutting boards and other tools used to treat samples before testing should be thoroughly cleaned and dried to avoid cross-contamination caused by residual liquid and impurities, which will affect the accuracy of testing.

2. It is forbidden to use spoiled bean and vegetable samples for testing; the large pieces of soil attached to the surface of the sample can be gently thrown off or wiped off with clean items to reduce impurity interference.

3. When sampling, priority is given to selecting the representative parts of beans and vegetables. The samples are fully chopped, stirred and mixed, and then weighed for testing to ensure that the test results truly reflect the actual drug residue of the samples.

4. The detection ambient temperature needs to be controlled at 17 to 30 degrees Celsius. Excessive or too low temperature will directly affect the immune chromatography reaction, resulting in deviations in the detection results.

5. Operate in strict accordance with the standard testing steps. Do not touch the color-developing area of the test strip during the test process, and avoid direct sunlight and direct blowing of the electric fan detection card.

6. The sample solution to be tested needs to be kept clear. The solution is turbid and contains suspended solids, which will cause problems such as inconspicuous color development and abnormal base color, which will interfere with the judgment of the result.

7. It is forbidden to use the test card for expired products and aluminum foil bags that are damaged and damp. The test card should be used immediately after unpacking to prevent damp failure.

8. After the sample processing is completed, it needs to be tested in time, and the sample needs to be reprocessed if it is placed for too long to avoid the volatilization and deterioration of the ingredients affecting the test results.

9. When the test has a positive result, it is recommended to retest; this product is only used for rapid primary screening, and the test results are for reference only. If accurate confirmation is required, it needs to be tested with reference to relevant national standards and methods.

10. When adding standards for verification, methanol is generally selected as the solvent for the standard solution, and the final sample dosage is recommended to be controlled at 10-50 μL.

11. When directly testing standards, use the kit's supporting extract as a solvent, and the amount of organic solvent added is controlled within 1%; tap water, distilled water, purified water or deionized water cannot be used as negative controls.

VIII. Safety instructions

1. To carry out experimental testing, it is necessary to be equipped with corresponding experimental equipment, and to standardize the wearing of necessary experimental equipment such as white clothes, gloves, and masks.

2. The test kit should be properly stored and placed in an area that is not easily accessible to children to prevent the risk of accidental touch and ingestion.

3. After the experiment, clean up the console in time to keep the laboratory clean and tidy to ensure air circulation in the experimental environment.

4. This product is a one-time testing consumable and cannot be reused; after the testing is completed, the experimental waste needs to be collected separately, and it is recommended to dispose of it according to the medical waste specification.

5. The supporting reagents of this product are safe and reliable, and do not contain carcinogenic, highly toxic, flammable, explosive, and strongly corrosive ingredients, but it is strictly prohibited to eat and avoid direct contact with the eyes, mouth, and nose.

Storage conditions and valid period

1. Original packaging: store in a dark, dry and cool environment at 4-30 ° C, and prohibit freezing. The product has a valid period of 12 months.

2. After unpacking: The test card needs to be used immediately after unpacking, and storage and freezing are prohibited.