Instructions

1 Principles and Uses of Vomitotoxin Colloidal Gold Rapid Detection Card

This product is made using the principle of competitive inhibition of colloidal gold immunochromatography for the detection of vomitotoxin residues in food, grain and feed samples. After the sample solution is dropped into the sample hole of the test card, the vomitotoxin in in the sample solution binds to the gold standard antibody, thereby preventing the gold standard antibody from binding to the vomitotoxin conjugate on the cellulose membrane. When the vomitotoxin content in the sample solution is greater than the detection limit, the detection line T does not show color, and the result is positive; when the vomitotoxin content in the sample solution is less than the detection limit, the detection line T shows purplish red, and the result is negative.

2 technical indicators

sample and methanol water dilution ratio

detection limit mg/kg

1: 9

1 ppm

1: 15

2 ppm

1: 20

3 ppm

1: 25

4 ppm

* If you need to detect higher concentrations, you can dilute

3 according to the corresponding proportion. You need to bring your own tool

balance, pipette, 15 ml low speed centrifuge, timer.

4 sample pretreatment

[Cereal and feed sample processing]

(feed and methanol water dilution ratio of 1:9)

4 The grain or feed sample to be tested is crushed into granules (do not grind into powder), weighed 1 g of sample in a 15 mL centrifuge tube;

4 Add 9 mL of 20% methanol water, mix violently for 1 minute, 4000 rpm Centrifuge for 3 minutes, supernatant as the liquid to be tested;

5 sample testing

5.1 Tear off the test card aluminum foil bag, take out the test card/gold standard microporous/dropper (a total of 3 kinds of consumables), put on a flat, clean table.

5 with pipette or aluminum foil bag matching dropper to absorb the above liquid to be tested 120 µL (about 4 drops) in the gold standard micropores, horizontal stand for 2 minutes, and then repeatedly blow the purple material in the micropores with a dropper until completely dissolved; all the liquid in the gold standard micropores is added dropwise to the sample well (S) of the detection card;

5.3 Start timing after adding the sample, and leave it at room temperature for 8-10 minutes to judge the result, and other time interpretation is invalid.

6 the results

negative: the control line (C) and the detection line (T) both appear purple lines, indicating that the concentration of vomiting toxin in the sample is lower than the detection limit or there is no vomiting toxin residue.

Positive: Purple red line appears on the control line C, and the detection line (T) does not show color, indicating that the concentration of vomiting toxin is higher than the detection limit.

Failure: Purple red line does not appear on the control line C.

7 Precautions

7 Products that have expired or the aluminum foil bag is damaged cannot be used.

7 When the test card is taken out of the refrigerator, it should be restored to room temperature and opened. The opened test card should be used as soon as possible to avoid moisture.

7 Do not touch the white film surface in the center of the test card.

7.4 The sample solution to be tested should be clear, free of cloudy particles, and free of bacterial contamination, otherwise it will easily lead to abnormal phenomena such as blockage and insignificant color development, which will affect the judgment of experimental results.

8 Storage and shelf life

8.1 Storage conditions: 4-30 ℃ Store in the dark, do not freeze.

8 Shelf life: 1 year, see the outer packaging for the production date.

9 kit composition

Specifications

Composition

10 times/box

20 times/box

Test card (containing gold standard micropores, droppers, desiccant)

10 parts

20 parts

20% methanol water

90mL/bottle 1 bottle

90mL/bottle 2 bottles

15ml centrifuge tube (recyclable)

2

4

Instructions

1 parts

1 parts