Melamine colloidal gold rapid detection card

instruction manual

Product number: YPAW-11

1 principle and use

This product is made of the principle of competitive inhibition colloidal gold immunochromatography for detecting melamine residues in milk samples (except yogurt). After the sample solution is dropped into the sample hole of the detection card, the melamine in the sample solution binds to the gold standard antibody, thereby preventing the gold standard antibody from binding to the melamine conjugate on the cellulose membrane. When the melamine content in the sample solution is greater than the detection limit, the detection line T does not show color, and the result is positive; when the melamine content in the sample solution is less than the detection limit, the detection line T shows purplish red, and the result is negative.

2 technical indicators

Detection sensitivity: 200 µg/kg (ppb)

3 Need to bring your own tools

electronic balance (0.1-100 g), pipette (0.1-1 ml), timer.

4 sample pretreatment

The temperature of the test environment should be about 25 ° C at room temperature, frozen raw milk, obvious particles, easy to lead to incomplete running plate, at this time must use heater heating or centrifugation to take the middle layer sample for testing.

5 sample test

5 Tear off the aluminum foil bag, take out the test card, and place it horizontally on the table;

5.2 Absorb the raw milk to be tested with a dropper, and add 4 drops (120 µL) vertically to the sample hole (S) of the test card;

5. 3 Start timing after adding the sample, and leave it at room temperature for 8-10 minutes to judge the result. Other results are invalid.

6 Results Judgment

Negative: Both the control line (C) and the detection line (T) appear purplish-red lines, indicating that the melamine concentration in the sample is lower than the detection limit or no melamine residue.

Positive: The control line (C) appears purplish-red lines, and the detection line (T) does not show color, indicating that the melamine concentration in the sample is higher than the detection limit.

Failure: In the detection window, the control line (C) does not appear purple line.

7 Precautions

7 Products that have expired or damaged aluminum foil bags should not be used.

7 When the test card is taken out of the refrigerator, it should be restored to room temperature and opened. The opened test card should be used as soon as possible to avoid failure after moisture.

7 Do not touch the white film surface in the center of the test card.

7 The liquid extraction dropper should not be mixed to avoid cross-contamination.

7.5 The sample solution to be tested should be clear, no cloudy particles, and no bacterial contamination, otherwise it will easily lead to abnormal phenomena such as blockage and inconspicuous color development, which will affect the judgment of experimental results.

8 Storage and storage period

8.1 Storage conditions: 4-30 ℃ Store in the dark, do not freeze.

8 Warranty period: The valid period of this product is 1 year, and the production date is shown in the outer packaging.

9 kit composition

specification

composition

10 times/box

20 times/box

test card (including dropper, desiccant)

10

20

sample cup

10

20

manual

1

1