Florfenicol Colloidal Gold Detection Card in Eggs Instructions for Use

1 Principles and Uses

Florfenicol Detection Card is used for rapid detection of florfenicol residues in poultry egg samples. This product is made using the principle of competitive inhibition colloidal gold immunochromatography and is used to detect florfenicol in samples. After the sample solution is dropped into the sample hole of the detection card, the florfenicol in the sample solution binds to the gold standard antibody, thereby preventing the gold standard antibody from binding to the florfenicol conjugate on the cellulose membrane. The result is judged according to the color development depth of the T-line and the C-line.

2 technical indicators, detection limit: 10 μg/kg (ppb)

3 tools

scissors, forceps, homogenizer, vortex mixer (optional), electronic balance, low speed centrifuge, pipette (0.1-1 mL), timer.

4 sample pretreatment

(1) Break the fresh eggs into the cup and mix well (egg white and egg yolk are well mixed);

(2) Absorb 0.2 g of the well-mixed egg sample into the detection tube, and then add 1.2 mL dilution;

(3) Vibrate violently for 1 minute or repeatedly suck the liquid in the detection tube with a dropper (not less than 10 times), which is the liquid to be tested;

5 sample testing

(1) Remove the test strip and gold label micropores from the original package, please use it as soon as possible after opening;

(2) Absorb 120 µL (about 4 drops) of the liquid to be tested with a pipette, add it vertically to the gold label micropores, and repeatedly suck with a dropper or pipette to dissolve the red substance in the micropores , wait for the reaction for 2 minutes;

(3) all the liquid in the suction micropores is added dropwise to the sample hole of the detection card;

(4) start timing after adding the sample, and leave it at room temperature for 8-10 minutes to determine the result, and the other time interpretation is invalid.

6 result judgment

Negative (-): T-line (detection line, near the end of the sample hole) color development is deeper than or equal to C-line (control line) color development, both indicating that the concentration of the substance to be tested in the sample is lower than the detection limit or no substance to be tested remains.

Positive (+): T-line color is lighter than C-line color, or T-line does not color, both indicating that the concentration of the test substance in the sample is higher than the detection limit; T-line is shallower than C-line, indicating that the concentration of the test substance in the sample is higher.

Invalid: Control line (C) does not appear purplish-red band, indicating that the operation process is incorrect or the test card has failed. In this case, you should read the instructions carefully again and re-test with a new test card. If the problem still exists, do immediately stop using this batch number of products and contact the supplier.

7 Precautions

(1) Please follow the operation steps to test. Do not touch the color-developing area of the test strip during operation. Avoid direct sunlight and direct blowing of electric fans during testing.

(2) This test strip is a disposable product, please do not reuse or mix the test card from different batches.

(3) When a positive result occurs, it is recommended to use this card for re-testing. The test results of this product are for reference only. If you need to confirm, please refer to the relevant national standards and methods.

(4) If you need to directly test the standard product, you need to use the special dilution in the kit to prepare.

(5) Please use the sample as soon as possible after processing, and the time process needs to re-process the sample for re-testing.

(6) Tap water, distilled water, purified water or deionized water cannot be used as a negative control.

8 Storage and shelf life

(1) Storage conditions: 4-30 ℃ Store in the dark, do not freeze.

(2) Shelf life: valid period 1 year, see the outer packaging for the production date.

9 kit composition

specification

composition

1 times/box

10 times/box

20 times/box

test card (containing gold standard micropores, droppers, desiccant)

1 parts

10 parts

20 parts

dilution

1 bottle

1 bottle

2 bottle

1 0.5 mL detection tube

1 pieces

10 pieces 11727798120 pieces

manual

1 parts

1 parts

1 parts

1 parts