Fipronil Colloidal Gold in Poultry Eggs

Instruction Manual

1 Principle and Use

Fipronil Detection Card is used for rapid detection of fipronil residues in poultry egg samples. This product is made by applying the principle of competitive inhibition of colloidal gold immunochromatography to detect fipronil in samples. After the sample solution is dropped into the sample hole of the detection card, the fipronil in the sample solution binds to the gold standard antibody, thereby preventing the gold standard antibody from binding to the fipronil conjugate on the cellulose membrane. The result is judged according to the color development depth of the T-line and the C-line.

2 technical specifications, detection limit: 0.02 mg/kg (ppm)

3 tools required

scissors, forceps, homogenizer, vortex mixer (optional), electronic balance, low speed centrifuge, pipette (0.1-1 mL), timer.

4 sample pretreatment

(1) Break the fresh eggs into the cup and mix well (egg white and egg yolk are well mixed);

(2) Absorb 0.2 grams of the well-mixed egg sample in the detection tube, and then add 1.2 mL dilution;

(3) Vibrate violently for 1 minute or repeatedly suck the liquid in the detection tube with a dropper (not less than 10 times), which is the liquid to be tested;

5 sample detection

(1) Tear open the aluminum foil bag of the detection card, take out the detection card/gold standard microporous/dropper (a total of 3 kinds of consumables), and place it on a flat and clean table.

(2) with a pipette or a dropper in an aluminum foil bag to absorb the above test solution 120 µL (about 4 drops) in the gold standard micropores, blow with a dropper until the purplish-red substance in the pores is completely dissolved, let stand horizontally, and wait for the reaction for 2 minutes; All the liquid in the gold standard micropores is added dropwise to the sample well (S) of the detection card; (3) Start timing after adding the sample, and the result is judged after 8-10 minutes at room temperature. The result interpretation for more than 10 minutes is invalid.

6 result judgment

Negative (-): T-line color development is deeper than C-line or there is no significant difference in color development with C-line, indicating that there is no substance to be tested in the sample or its concentration is lower than the detection limit.

Positive (+): The color development of the T-line is significantly weaker than that of the C-line or the T-line does not develop color, indicating that the concentration of the substance to be tested in the sample is equal to or higher than the detection limit.

Invalid: No C-line appears, indicating that the operation process is incorrect or the test card has failed. In this case, the instructions should be read carefully again and re-tested with a new test card.

7 Precautions

(1) Products that have expired or damaged aluminum foil bags should not be used.

(2) The test card should be restored to room temperature and opened when taken out of the refrigerator. The opened test card should be used as soon as possible to avoid failure.

(3) Do not touch the white film surface in the center of the test card.

(4) The liquid extraction dropper should not be mixed to avoid cross-contamination.

(5) The liquid to be tested should be clear, no cloudy particles, and no bacterial contamination, otherwise it will easily lead to abnormal phenomena such as blockage and inconspicuous color development, which will affect the judgment of experimental results.

(6) Please store the reagent in a place that is not easy for children to contact, and do not eat the reagent.

(7) If you need to directly test the standard product, please prepare it with the diluent provided in the kit.

8 Storage and shelf life

(1) Storage conditions: 4-30 ° C Store in the dark, do not freeze.

(2) Shelf life: valid period 1 year, see the outer packaging for the production date.

9 kit composition

specification

composition

1 times/box

10 times/box

20 times/box

detection card (containing gold standard micropores, droppers, desiccant)

1 parts

10 parts

20 parts

dilution

1 bottle

1 bottle

1 bottle

2 bottle

1 mL detection tube

1 pieces

10 pieces

20 pieces

20 pieces

1 mL straw

1 pieces

10 pieces

20 pieces

manual

1 parts

1 parts

1 parts 117277984