Fipronil Colloidal Gold Rapid Detection Card Instruction Manual

1 Principle and Use

This product is made of the principle of competitive inhibition colloidal gold immunochromatography for the detection of fipronil pesticide residues in vegetables and fruits. After the sample solution is dropped into the sample hole of the detection card, the fipronil in the sample solution binds to the gold standard antibody, thereby preventing the gold standard antibody from binding to the fipronil conjugate on the cellulose membrane. The detection result is judged by the color depth of C and T lines.

2 technical indicators, detection limit: 0.02 mg/kg (ppm)

3 You need to bring your own tools

electronic balance (0.1-100 g), scissors, forceps, disposable cups, vortex mixer, pipette (0.1-1 ml), timer.

4 sample pretreatment

4 Take an appropriate amount of sample, cut about 1 cm square, weigh 1 gram of shredded sample in the sample cup with lid, add 1 ml of diluent, fully shake for 2 minutes, let stand for 1 minute, as the liquid to be tested;

4 .2 Take 1 1.5 mL centrifuge tube, dilute the sample solution with the diluent in different proportions according to the table, and the diluted solution is the liquid to be tested;

Different sample dilution ratios and detection limits

Sample name

Sample treatment solution

Fipronil dilution

Fruits and vegetables (20 ppb)

120 μL

0 μL

Fresh corn (100 ppb)

100 μL11727798400μL

5 Sample detection

(1) Tear open the aluminum foil packaging bag of the detection card and take out the detection card/gold standard micropore/dropper ( Material), placed on a flat, clean countertop.

(2) with a pipette or aluminum foil bag matching dropper to absorb the liquid to be tested 120 µL (about 4 drops) in the gold standard micropores, blown with a dropper to completely dissolve the purplish-red material in the pores, let stand horizontally, and wait for the reaction for 2 minutes; all the liquid in the gold standard micropores is added dropwise to the sample well (S) of the detection card;

(3) Start timing after adding the sample, and leave it at room temperature for 8-10 minutes to judge the result, and the other time interpretation is invalid.

6 The result is judged

negative (-): T-line color development is deeper than C-line or there is no significant difference with C-line color development, indicating that there is no substance to be tested in the sample or its concentration is lower than the detection limit.

Positive (+): The color development of the T-line is significantly weaker than that of the C-line or the T-line does not develop color, indicating that the concentration of the substance to be tested in the sample is equal to or higher than the detection limit.

Invalid: No C-line appears, indicating that the operation process is incorrect or the test card has failed. In this case, the instructions should be read carefully again and re-tested with a new test card.

7 Precautions

(1) Products that have expired or damaged aluminum foil bags should not be used.

(2) The test card should be restored to room temperature and opened when taken out of the refrigerator. The opened test card should be used as soon as possible to avoid failure.

(3) Do not touch the white film surface in the center of the test card.

(4) The liquid extraction dropper should not be mixed to avoid cross-contamination.

(5) The liquid to be tested should be clear, free of cloudy particles, and free of bacterial contamination, otherwise it will easily lead to abnormal phenomena such as blockage and inconspicuous color development, which will affect the judgment of experimental results.

(6) Please store the reagent in a place that is not easy for children to contact, and do not eat the equipped reagent.

(7) If you need to directly test the standard product, please prepare it with the diluent provided in the kit.

8 Storage and shelf life

(1) Storage conditions: 4-30 ° C Store in the dark, do not freeze.

(2) Shelf life: 1 year, see the outer packaging for the production date.

9 kit composition

specification

composition

10 times/box

20 times/box

test card (containing gold standard micropores, dropper, desiccant)

10 parts

20 parts

fipronil dilution

1 bottle

2 bottle

sample cup with lid

1 pieces

2 pieces

1 ml straw

10 pieces

20 pieces

1 0.5 mL centrifuge tube

10 pieces

20 pieces

manual

1 parts

1 parts

1 parts