Enrofloxacin Residue Colloidal Gold Rapid Detection Card Instructions for Use Product Number: YB117R01K SUMMARY Enrofloxacin (ENR) belongs to the fluoronolone class of highly effective antimicrobial drugs and is widely used in the prevention and treatment of animal diseases in animal husbandry and aquaculture. It has attracted extensive attention due to its drug resistance and potential carcinogenicity. With the large-scale and irregular application of enrofloxacin in breeding, its residue problems in animal foods such as livestock and poultry and aquatic products have also been paid more and more attention. This product is suitable for enterprises, testing institutions, supervision departments and other types of on-site rapid detection. Detection Principle This product is made using the principle of competitive inhibition of colloidal gold immunochromatography and is used to detect enrofloxacin residues in poultry egg samples. After the sample solution is dropped into the sample hole of the detection card, the enrofloxacin in the sample solution binds to the gold-standard antibody, thereby preventing the gold-standard antibody from binding to the enrofloxacin conjugate on the cellulose membrane. According to the color development depth judgment results of the T-line and the C-line. Scope of application This product is suitable for the qualitative detection of enrofloxacin drugs in fresh egg samples such as eggs and duck eggs. Note: The type of detection sample refers to the national standard GB2763-2021. Detection limit 10 ppb (μg/kg) Kit composition Serial number Specification composition 10 times/box 20 times/box (1) Detection card (containing dropper, desiccant) 10 parts 20 parts (2) 1 bottle of dilution 2 bottles (3) 7mL centrifuge tube 10 pieces 20 pieces (4) 1.5 mL detection tube 10 pieces 20 pieces (5) 0.3 mL straw 10 pieces 20 pieces (6) Instruction manual 1 part 1 part Precautions (1) Before each sample, the knife for shredding the sample needs to be cleaned to avoid cross-contamination. ( 2) The test environment temperature should be controlled at 18~ 30 ℃. If the temperature is too high or too low, it will affect the test results. (3) Please follow the test steps to test. Do not touch the color-developing area of the test strip during operation to avoid direct sunlight and direct blowing of electric fans. (4) Please use the sample as soon as possible after processing. If the time is too long, you need to re-process the sample for re-testing. (5) The sample solution to be tested needs to be clarified, otherwise it will easily lead to abnormal phenomena such as inconspicuous color development, which will affect the judgment of experimental results. (6) Products that have expired or damaged aluminum foil bags cannot be used. Please use the test card immediately after unpacking. (7) This product is a one-time product. Please do not reuse or mix test cards from different batches. ( 8) When a positive result appears, it is recommended to retest. The test results of this product are for reference only. If you need to confirm, please refer to the relevant national standard methods. (9) When evaluating the product, if you need to directly test the standard product, you need to use the special dilution solution in the kit to prepare. (10) Tap water, distilled water, purified water or deionized water cannot be used as a negative control. Safety instructions (1) The experiment needs to match the corresponding experimental equipment and wear the necessary experimental equipment (white clothes, gloves, masks, etc.). (2) The test kit needs to be kept in a place that is not easy for children to come into contact with. (3) All items used in the experiment should be properly disposed of after application. (4) After the experiment, keep the laboratory clean and tidy and the air flow of the experimental environment. ( 5) Experimental waste is collected separately, and it is recommended to dispose of it according to medical waste. (6) Do not eat the reagents provided. Storage conditions and valid period (1) Storage conditions: 2-30 ° C Store in the dark, do not freeze. (2) Valid period: 12 months.