Today, with the increasing attention paid to the quality and safety of agricultural products, green onions are a common vegetable on the table, and their pesticide residues have always been the focus of consumers' attention. Thiamethoxam, as an efficient and broad-spectrum neonicotinoid insecticide, is often used for pest control in green onions, but excessive or unreasonable use may lead to its residue in green onions, affecting food safety. In order to quickly and accurately detect the residue of thiamethoxam in green onions, a rapid detection card for colloidal gold in green onions came into being.
This detection card is a rapid detection product developed based on immunochromatography technology. It is mainly composed of sample pad, binding pad, nitrocellulose membrane (detection line and quality control line) and absorbent paper. The core principle is to use the colloidal gold-labeled thiamethoxam antibody to generate an immune competition reaction with the thiamethoxam antigen in the sample. When the sample is added dropwise to the sample well of the detection card, it will flow through the binding pad and nitrocellulose membrane in sequence along the chromatography path. If the sample does not contain thiamethoxam or the residue of thiamethoxam is low, the colloidal gold-labeled antibody will bind to the antigen on the detection line (coated with thiamethoxam conjugate), and red bands will appear on the detection line and the quality control line; conversely, if the residue of thiamethoxam in the sample is high, it will compete with the detection line antigen to bind the antibody, resulting in the color of the detection line becoming lighter or disappearing, and only
In practical applications, the detection card is extremely simple to operate, no complicated instruments are required, just drop the processed green onion sample (such as homogenate, extract) into the sample hole, and wait 5-15 minutes to read the results. It can quickly provide preliminary screening data for agricultural product regulatory departments, planting enterprises, and market quick inspectors. Compared with traditional detection methods (such as high-performance liquid chromatography), it has the advantages of fast detection speed, low cost, and strong portability. It can be used as an ideal tool for large-scale rapid screening, helping to detect and control the risk of thiamethiazide residues in green onions in time, and build a first line of defense to ensure the "safety on the tip of the tongue" of consumers.