What is the enrofloxacin colloidal gold rapid detection card?

2025-08-18

Enrofloxacin colloidal gold rapid detection card is a rapid detection product based on colloidal gold immunochromatography technology in the field of food safety testing. It is mainly used for qualitative or semi-quantitative detection of enrofloxacin residues in foods (such as livestock and poultry meat, aquatic products, dairy products, etc.).

From a technical principle, the detection card uses the immune response principle of antigen-antibody specific binding: enrofloxacin antigen is fixed on the detection line (T-line), and the colloidal gold-labeled enrofloxacin antibody is pre-coated on the binding pad. During testing, the sample solution is dropped into the sample well and moved along the test strip under capillary action. If the sample contains enrofloxacin, it will bind to the colloidal gold-labeled antibody to form a complex. When the complex passes through the detection line, if the concentration of enrofloxacin in the sample reaches the detection limit, the complex will compete with the antigen of the T-line to bind the colloidal gold-labeled antibody, resulting in the color development of the T-line. If the sample does not contain enrofloxacin, the T-line will not develop color. By comparing the color development with the quality control line (C-line), the detection result can be judged.

Compared with traditional detection methods (such as high-performance liquid chromatography, mass spectrometry, etc.), the enrofloxacin colloidal gold rapid detection card has significant advantages: the detection speed is fast, usually 10-15 minutes to produce results; the operation is simple, no professional instruments and complex pretreatment are required, and ordinary personnel can complete it with simple training; high sensitivity can reach ng/mL level; low cost, suitable for large-scale screening.

In the application scenario, it is widely used in food production enterprises of raw materials into the factory testing, quality monitoring in the process of processing, as well as the market supervision department of the circulation of food rapid sampling, can also be used for third-party testing agencies batch sample screening, help timely detection of enrofloxacin residues exceed the standard of food, from the source to control food safety risks, to ensure consumer food safety.