Ciprofloxacin Colloidal Gold Rapid Detection Card is a rapid detection product based on colloidal gold labeling immunochromatography technology. It is mainly used for the residue detection of the antibiotic ciprofloxacin in food. As a broad-spectrum antibiotic, ciprofloxacin is often used to prevent and treat bacterial infections in livestock and poultry farming, but excessive residues may endanger human health, so it is essential to quickly and accurately detect its content in food.
The core principle of this detection card is to utilize antigen-antibody specific binding reaction: After the colloidal gold-labeled antibody binds to the ciprofloxacin antigen, it binds to the coated ciprofloxacin-carrier protein on the nitrocellulose membrane (NC membrane) by chromatography to form a visible color-developing band. If the sample contains ciprofloxacin, it will compete with the colloidal gold-labeled antibody to bind the coating antigen, causing the color of the detection line to become lighter or disappear, so as to determine whether there is residue.
Its structure usually includes sample pad, binding pad (containing colloidal gold-labeled antibody), NC membrane (coating detection line and quality control line), absorbent pad and PVC baseplate. When using, just add the sample (such as meat, milk, etc.) to the sample well after processing, wait for a few minutes, and interpret the result by observing the color development of the detection line and quality control line. The whole process usually takes 10-20 minutes, no complicated equipment is required, and it is easy to operate.
Compared with traditional detection methods (such as high-performance liquid chromatography, mass spectrometry, etc.), the ciprofloxacin colloidal gold rapid detection card has significant advantages: fast detection speed, suitable for on-site rapid screening; simple operation, no professional training required; low cost, suitable for large-scale sample detection; high sensitivity, which can reach ng-level detection limit; and fewer sample pre-processing steps, reducing errors caused by complex operations.
At present, the detection card is widely used in food safety supervision, aquaculture quality control, import and export inspection and quarantine and other fields, which can effectively help regulatory authorities and enterprises to quickly identify the risk of ciprofloxacin residues, and provide important technical support for ensuring public dietary safety. With the continuous optimization of technology, its detection accuracy and stability are also continuously improving, becoming an important tool in the food safety rapid detection system.