Chlorpromazine colloidal gold rapid detection card is a rapid screening product based on colloidal gold immunochromatography technology in the field of food safety detection. It is mainly used to detect the residue of chlorpromazine in food. It achieves rapid qualitative or semi-quantitative analysis of the target substance in the sample through antigen-antibody specific binding reaction, combined with the color development characteristics of colloidal gold particles.
From the technical principle, the core of the detection card is to use chlorpromazine antigen to combine with colloidal gold-labeled antibodies to form a complex. When the sample (such as meat, dairy products, aquatic products, etc.) is added dropwise to the loading well of the detection card, the chlorpromazine in the sample (if present) will compete with the colloidal gold-labeled antibody to bind the detection line-coated chlorpromazine-carrier protein conjugate. If the concentration of chlorpromazine in the sample reaches the detection threshold, the colloidal gold-labeled antibody cannot bind to the detection line, and the detection line does not show color; conversely, the detection line shows color, indicating that the sample does not contain or contains a small amount of chlorpromazine. At the same time, the quality control line always shows color as the basis for judging the validity of the detection card.
Structurally, the chlorpromazine colloidal gold rapid detection card is usually composed of a sample pad, a binding pad (containing colloidal gold-labeled antibodies), an NC film (detection line and quality control line), absorbent paper, and a plastic shell. When using, just drop the processed sample into the sample hole, wait for a period of time (usually 10-20 minutes), and judge the result by observing the color rendering of the detection line and the quality control line. The whole process does not require complex instruments, and the operation is convenient.
As a rapid detection tool, it is widely used in food production enterprises for raw material acceptance, processing process monitoring, finished product quality check, as well as on-site sampling screening, laboratory preliminary screening and other scenarios of market supervision departments. Compared with traditional detection methods (such as high performance liquid chromatography, mass spectrometry, etc.), it has the advantages of fast detection speed (results within 15 minutes), simple operation (no professional training required), low cost (only a few yuan for a single test), and high throughput (multiple samples can be detected at the same time), which can effectively help enterprises and regulatory authorities to quickly identify the risk of chlorpromazine exceeding the standard and ensure food safety.
However, the test results of the test card are only used as a preliminary screening basis. If accurate quantification is required, it needs to be further confirmed in combination with laboratory instrument analysis. Overall, the chlorpromazine colloidal gold rapid test card plays an important role in the rapid detection of food safety due to its high efficiency and convenience. It is one of the important technical means to protect the public's dietary safety.