Instruction manual for histamine quick test box in aquatic products

YC171R01H_ aquatic products histamine rapid test box _ colorless card _100 times.pdf

In aquatic products
Histamine Quick Test Kit Instruction Manual

                                    
Product number:
YC171R01H

1简介

  
Histamine is a potentially harmful nitrogen-containing low molecular weight organic compound that is widely present in various foods and is easy to produce in large quantities in red meat fish represented by tuna. Excessive intake of histamine can cause harm to the human body, and eating certain fish or other animals containing a certain amount of histamine can cause allergic food poisoning. Histamine poisoning is characterized by euphoria, mild symptoms, quick recovery, and short incubation period, manifested as mental weakness, allergies, skin flushing, vomiting, diarrhea, etc.

2Limited standard

  
GB2733-2015
"Fresh and Frozen Animal Aquatic Products"
Provisions:
High histamine fish
≤40 
mg/100g
Other marine fish
≤20 
mg/100g
。

3检测原理  

After histamine is extracted, it reacts with the detection reagent to form a colored compound. The color depth is related to the histamine content, and the approximate content can be obtained by comparing with the color card.

4检测范围  fish

5技术指标  Lower detection limit:
5 
mg/100g
；检测范围：0-100 
mg/100g

6样品处理及测定

6.1实验前取试剂F
Pour into the reagent
G
Mix well as a reaction reagent.

6.2称取2 
g
粉碎后的鱼肉于7 
mL
离心管中，加试剂A
3 
mL
，剧烈振摇5分钟，静置5分钟后取上清作为待测液，必要时过滤或离心。

6.3取待测液0.5 
mL
于10 
mL
离心管中，加入1滴试剂B
Make the liquid alkaline, then add
1 
mL
试剂C
, shaking violently
5分钟后于4000 
r/min
离心2分钟，上清作待测液。

6.4取0.5 
mL
上清于另一7 
mL
离心管中，再加入1 
mL
试剂D
, shaking violently
5分钟，静置分层后取下层作为待测液。

6.5取下层液体0.5 
mL
于2 
mL
离心管中，加入1 
mL
试剂E
Mix well and add the reagent
F
With reagents
G
mixture of
0.5 
mL
，摇匀。静置5分钟后与色卡比对，颜色相近的色阶即为样本中组胺的实际浓度。

7注意事项

7.1The experimental water is purified water or distilled water.

7.2The experiment should be carried out in a ventilated environment. If it comes into contact, please rinse it with plenty of water immediately.

7.3This product is only used for initial screening, and the final result is subject to the relevant national standards and methods.

8储存条件与有效期 

  
试剂在2-8℃
Store in a cool and dry place away from light, the valid period is
12个月。

9试剂盒装箱单

                                                                
规格： 
100time
/
盒