Chlorothalonil Colloidal Gold Rapid Test Card Instruction Manual

Chlorothalonil Colloidal Gold Rapid Detection Card Instruction Manual

Product Number: YB097C01K

Summary

Chlorothalonil is a broad-spectrum and protective fungicide. The mechanism of action is to destroy the activity of glyceraldehyde triphosphate dehydrogenase in fungal cells, so that the metabolism of fungal cells is damaged and lost vitality. It is mainly used for the prevention and control of rust, anthrax, powdery mildew and downy mildew on fruit trees and vegetables.

Detection Principle

This product is made of the principle of competitive inhibition of colloidal gold immunochromatography for the detection of chlorothalonil pesticide residues in vegetables and fruits. After the sample solution is dropped into the sample hole of the detection card, the chlorothalonil in the sample solution is combined with the gold standard antibody, thereby preventing the gold standard antibody from binding with the chlorothalonil conjugate on the cellulose membrane, and the color depth of the C and T lines is used to judge the detection result.

Scope of application

This product is suitable for qualitative detection of chlorothalonil residues in fresh vegetables, fruits and other samples.

Note: The type of test sample refers to the national standard GB2763-2021.

detection limit

0 mg/kg (ppm)

kit composition

need to bring your own tools

serial number

specification
composition

10 times/box

20 times/box

serial number

tool name

(1)

test card (containing dropper, desiccant)

10 parts

20 parts

(1)

balance (accuracy 0.01g)

(6)

vortex meter

(2)

chlorothalonil dilution

1 bottle

2 bottle

(2)

scissors

(7)

timer

(3)

20 mL graduated sample cup

1 pieces 11727798001 mL centrifuge tube

10

20

(5)

pipette (0.2mL/1mL)

(6)

Instruction

1 parts

1 parts

precautions

(1) Tools such as knives, scissors, and stickers for pre-testing samples should be cleaned to avoid cross-contamination.

(2) Sample requirements: avoid spoilage and deterioration of samples; avoid large pieces of soil (can be thrown off or erased with other clean items).

(3) Before testing, it is recommended that the sample be fully stirred and mixed (if there is less sampling, a representative part should be taken and then weighed), so that the test results can more accurately reflect the actual drug residue of the sample.

(4) The test environment temperature should be controlled at 20~ 30 ℃. If the temperature is too high or too low, it will affect the test results.

(5) Please follow the test steps for testing. Do not touch the color-developing area of the test strip during operation. Avoid direct sunlight and direct blowing of electric fans.

(6) The sample solution to be tested needs to be clarified, otherwise it will easily lead to abnormal phenomena such as inconspicuous color development, which will affect the judgment of experimental results.

(7) Products that have expired or damaged aluminum foil bags should not be used. Please use the test card immediately after unpacking.

(8) Please use the sample as soon as possible after processing. If the time is too long, you need to reprocess the sample for re-testing.

(9) When a positive result appears, it is recommended to re-test. The test results of this product are for reference only. If you need confirmation, please refer to the relevant national

(10) When adding standard for verification, methanol is generally selected for the solvent of the standard solution, and the final sample is recommended to be 10-50 μL.

(11) When directly testing the standard product, the solvent should be used to match the extraction solution of the kit, and the amount of organic solvent should be controlled within 1%. Tap water, distilled water, purified water or deionized water cannot be used as a negative control.

Safety instructions

(1) The experiment should match the corresponding experimental equipment and wear the necessary experimental equipment (white clothes, gloves, masks, etc.).

(2) The detection kit should be properly stored and placed in a place that is not easy for children to contact.

(3) Keep the laboratory clean and the air flow of the experimental environment after the experiment.

(4) This product is a one-time product, should be properly disposed of after testing, experimental waste collected separately, it is recommended to treat according to medical waste.

(5) The reagents involved in this product are safe and reliable, do not contain carcinogenic, highly toxic, flammable, explosive, strong corrosive reagents, but do not eat.

storage conditions and valid period

(1) storage conditions: 2-30 ℃ protected from light, do not freeze.

(2) valid period: 12 months.