Pyrazole ether mycorrhizal ester colloidal gold rapid detection card instruction manual

Product number: YB098C01K

1 principle and use

This product applies the principle of competitive inhibition colloidal gold immunochromatography for the detection of pyrazole ether mycorrhizal ester residues in vegetables and fruits. After the sample solution is dropped into the sample hole of the detection card, the pyrazole ether mycorrhizal ester in the sample solution binds to the gold standard antibody, thereby preventing the gold standard antibody from binding to the pyrazole ether mycorrhizal ester conjugate on the cellulose membrane. The detection result is judged by the color depth of the C and T lines.

2 technical specifications, minimum detection limit: 0.2 mg/kg (ppm)

3 Required tools

electronic balance (0.1-100 g), scissors, forceps, disposable cups, vortex mixer (optional), pipette (0.1-1 mL), timer.

4 sample pretreatment 117277984Iv.1 Samples need to be restored to room temperature (20-30 ° C) before testing.

4 Take an appropriate amount of sample, cut about 1 cm square, weigh 2 grams of chopped sample in the sample cup, add 3 mL dilution, fully soak and shake for 2 minutes, let stand for 1 minute, as the sample liquid;

4 Take 1 1.5 mL centrifuge tube, the sample liquid and the dilution liquid are diluted in different proportions according to the table, and the diluted solution is the liquid to be tested;

Different sample dilution ratios and detection limits

Sample name

Dilution ratio

(sample liquid: dilution liquid)

National standard limited GB2763-2021

miscellaneous grains (except mung beans, peas, lentils)

No dilution, direct use (1:0)

0 mg/kg

Peas, Brussels sprouts, eggplant, winter squash

500 µL: 250 µL

(1:0.5)

0 mg/kg117277984 Brussels sprouts, nightshade vegetables (except tomatoes and eggplants), radishes, carrots, lentils, kumquats, apples

2 00 µL: 300 µL

(1:1.5)

0 mg/kg

Kale, cauliflower, tomato zucchini, loofah, peach, dates

1 00 µL: 400 µL

(1:4)

1 mg/kg

Citrus fruits (except tangerines, oranges, lemons, pomelos and kumquats), day lily

100 µL: 900 µL

(1:39)

2 mg/kg

Bitter gourd, green onion, tangerine, orange, loquat, apricot, cherry, blackberry 11727798150 µL: 750 µL

(1:15)

3 mg/k (1:24)

5 mg/kg

Leaf mustard, radish leaf, canola

20µL: 1480µL

(1:74)

15 mg/kg

5 Sample testing

5.1 Tear the test card aluminum foil packaging bag, remove the test card, gold label micropores and dropper, and place it on a flat, clean countertop.

(2) Pipette 120µL of the liquid to be tested in the gold standard micropores, slowly aspirate to dissolve the red substance in the micropores, and wait for the reaction for 2 minutes;

(3) Pick up all the mixed liquid in the micropores, and add it vertically dropwise to the sample hole (S hole) of the detection card;

(4) Start timing after adding the sample, leave it at room temperature for 5-8 minutes, according to the schematic judgment result, other times are determined to be invalid.

6 result Judgment

Negative (-): T-line color development is deeper than C-line or there is no significant difference from C-line color development, indicating that there is no substance to be tested in the sample or its concentration is lower than the detection limit.

Positive (+): The color development of the T-line is significantly weaker than that of the C-line or the T-line does not develop color, indicating that the concentration of the substance to be tested in the sample is equal to or higher than the detection limit.

Invalid: The C-line does not appear, indicating that the operation process is incorrect or the test card has failed. In this case, the instructions should be read carefully again and re-tested with a new test card.

7 Precautions

7 Products that have expired or the aluminum foil bag is damaged should not be used.

7 When the test card is taken out of the refrigerator, it should be restored to room temperature and opened. The opened test card should be used as soon as possible to avoid failure after being damp.

7 Do not touch the white film surface in the center of the test card.

7.4 The liquid extraction dropper should not be mixed to avoid cross

7 The sample solution to be tested should be clear, free of cloudy particles, and free of bacterial contamination, otherwise it will easily lead to abnormal color development and other phenomena, which will affect the result judgment.

8 Storage and shelf life

8 Storage conditions: 4-30 ° C Store in the dark, do not freeze.

8.2 Shelf life: valid period 1 year, see the outer packaging for the production date.

9 kit composition

Specifications

Composition

10 times/box

20 times/box

Test card (containing gold standard micropores, droppers, desiccants)

10 parts

20 parts

Pyrazole ether mycosteryl ester dilution

1 bottles

2 bottles

20 mL graduated sample cup (recyclable)

1 pieces

2 pieces

1 mL centrifuge tube