Chlorpyrifos Colloidal Gold Quick Detection Card Instruction Manual

Product Number: YB085C01K

1 Principle and Use

This product applies the principle of competitive inhibition colloidal gold immunochromatography to detect chlorpyrifos residues in vegetables and fruits. After the sample solution is dropped into the sample hole of the detection card, the chlorpyrifos in the sample solution binds to the gold standard antibody, thereby preventing the gold standard antibody from binding to the chlorpyrifos conjugate on the cellulose membrane. The detection result is judged by the color depth of C and T lines.

2 technical indicators, the minimum detection limit: 0.02 mg/kg (ppm)

3 need to bring your own tools

electronic balance (0.1-100 grams), scissors, forceps, disposable cups, vortex mixer, pipette, timer.

4 sample pretreatment 11727798400.1 The sample needs to be restored to room temperature (20-30 ° C) before testing. Take an appropriate amount of sample, cut about 1 cm square, weigh 2 grams of chopped sample in a 15 mL centrifuge tube, add 3 mL of dilution, shake and mix manually (about 50 times/minute) 2 minutes, let stand for 1 minute, to obtain the sample solution;

4 Take 1 1.5 mL centrifuge tube, dilute the sample solution with the dilution in different proportions according to the table, the diluted solution is the liquid to be tested;

Different sample dilution ratios and detection limits

Sample name

Dilution ratio

(sample solution: dilution)

National standard limited GB2763-2021

vegetables (except celery, asparagus, artichoke)

No dilution

(1:0)

0 mg/kg

Celery, asparagus, artichoke

1200 µL: 300 µL (1: 1.5)

0 mg/kg

Strawberry

1 00 µL: 1400 µL (1:14)

0 mg/kg

Orange, orange, kumquat, apple, pear, hawthorn, loquat

2 0 µL: 980 µL (1:49)

1 mg/kg

Orange, lemon, pomelo, kiwi fruit, banana

10 µL: 990 µL (1:99)

2 mg/kg

5 Sample testing

5. 1 Tear the test card aluminum foil packaging bag, take out the test card/gold label micropore/dropper (a total of 3 consumables), and place it on a flat and clean countertop.

5.2 Use a pipette or a matching dropper in an aluminum foil bag to absorb the above-mentioned 120µL (about 4 drops) of the liquid to be tested in the gold label micropores, blow with a dropper until the purplish-

6 result judgment

Negative (-): The color development of T-line is darker than that of C-line or there is no significant difference from that of C-line, indicating that there is no substance to be tested in the sample or its concentration is below the detection limit.

Positive (+): The color development of T-line is significantly weaker than that of C-line or T-line does not develop color, indicating that the concentration of the substance to be tested in the sample is equal to or higher than the detection limit.

Invalid: No C-line appears, indicating that the operation process is incorrect or the test card has failed. In this case, the instructions should be read carefully again and re-tested with a new test card.

7 Precautions

7.1 Products that have expired or the aluminum foil bag is damaged should not be used

7 When the test card is taken out of the refrigerator, it should be restored to room temperature and opened. The opened test card should be used as soon as possible to avoid dampness.

7 Do not touch the white film surface in the center of the test card.

7 The liquid dropper should not be mixed to avoid cross-contamination.

7. 5 The sample solution to be tested should be clear, no cloudy particles, no bacterial contamination, otherwise it will easily lead to abnormal color development and other phenomena, which will affect the result judgment.

8 Storage and shelf life

8 Storage conditions: 4-30 ℃ in the dark, do not freeze.

8.2 Shelf life: valid period 1 year, see the outer packaging for the production date.

9 kit composition

specification

composition

10 times/box

20 times/box

test card (containing gold standard micropores, dropper, desiccant)

10 parts

20 parts

chlorpyrifos dilution

1 bottle

2 bottle

15 mL centrifuge tube

1 pieces

2 pieces

1 pieces

1 mL centrifuge tube

10 pieces

20 pieces

manual

1 parts

1 parts