Instruction Manual

1 Principle and Usage of Carbonarb Colloidal Gold Rapid Detection Card

This product is made by applying the principle of competitive inhibition of colloidal gold immunochromatography for the detection of carbonarb residues in vegetables and fruits. After the sample solution is dropped into the sample hole of the detection card, the carbonarb in the sample solution is combined with the gold standard antibody, thereby preventing the gold standard antibody from binding to the carbonarb conjugate on the cellulose film. The detection result is judged by the color depth of the C and T lines.

2 Technical indicators

Detection sensitivity: 500 µg/kg (ppb)

3 kit composition

test card (containing gold standard micropores, droppers, desiccant) 10; 1 bottle of methanazole dilution; 10 sample cups;

Instructions 1 copy

4 need to bring your own equipment and reagents 117277984iron.1 Instrument: balance (sensitivity 0.01g) 117277984iron.2 Micropipette: single channel 20µL-200µL, 100µL-1000µL

5 Sample pretreatment

Take an appropriate amount of sample, cut it into 2-3 mm length, weigh the shredded sample 1.0 g in the sample cup, add 1 ml of methanazole dilution, fully soak and shake for 2 minutes, pending inspection;

6 sample testing

6.1 Tear off the aluminum foil bag, take out the test card, and place it horizontally On the table;

6 with a dropper to absorb the sample solution to be tested, add 4 drops (about 120 µL) to the gold standard micropores vertically, and then use the dropper to fully mix the red substance in the micropores and the solution to be tested, and let stand for 2 minutes;

6 All the liquid in the suction micropores is added dropwise to the sample well of the detection card (S);

6 Start timing after adding the sample, and leave it at room temperature for 10-15 minutes to judge the result, other results are invalid.

7 The result is judged

negative: both the control line (C) and the detection line (T) appear purplish-red lines, indicating that the concentration of carbonavil in the sample is lower than the detection limit or there is no carbonavil residue.

Positive: Purple red line appears on the control line (C), and the detection line (T) does not show color, indicating that the concentration of carbamide in the sample is higher than the detection limit.

Failure: Purple red line does not appear on the control line (C).

8 Precautions

8 Products that have expired or the aluminum foil bag is damaged cannot be used.

8 When the test card is taken out of the refrigerator, it should be restored to room temperature and opened. The opened test card should be used as soon as possible to avoid moisture.

8 Do not touch the white film surface in the center of the test card.

8 The liquid extraction dropper should not be mixed to avoid cross-contamination.

8.5 The sample solution to be tested should be clear, free of cloudy particles, and free of bacterial contamination, otherwise it will easily lead to abnormal phenomena such as blockage and insignificant color development, thus affecting the judgment of experimental results.

8. 6 If you need to directly test the standard product, you need to prepare the dilution of carboxyl in the kit.

9 Storage and shelf life

9 Storage conditions: 4-30 ℃ in the dark, do not freeze.

9.2 Shelf life: valid period 1 year, see the outer packaging for the production date.