What is the pentazole colloidal gold rapid detection card?

In agricultural production, as a highly effective fungicide, pentazole alcohol is widely used in disease control of wheat, rice, fruits and other crops, which can effectively reduce the impact of pests and diseases on yield and quality. However, excessive use or failure to observe the safety interval before harvest may lead to excessive pentazole alcohol residues in agricultural products, threatening human health. Therefore, rapid and accurate detection of pentazole alcohol residues in agricultural products is essential to ensure food safety. And the pentazole colloidal gold rapid detection card is an important tool to meet this demand.

So, what exactly is the pentazole colloidal gold rapid detection card? It is a rapid detection product based on colloidal gold immunochromatography technology. The colloidal gold immunochromatography technique is to bind the colloidal gold-labeled antibody to the antigen, and form a color-developing strip on the test strip through chromatography, so as to realize qualitative or semi-quantitative detection of the target. Specifically, the detection card contains the sample pad, binding pad, nitrocellulose membrane (NC membrane) and absorbent paper. When the sample (such as agricultural product extract) is added dropwise to the detection card and the sample well, it will flow through each area in turn through chromatography. If the sample contains pentazolol, it will compete with the pentazolol-carrier protein conjugate labeled with colloidal gold on the binding pad to bind the pre-coated pentazolol antigen on the NC membrane. If the concentration of pentazolyl alcohol in the sample reaches the detection limit, the combination cannot fully bind to the antigen, and the detection line (T-line) will develop color; conversely, when the combination binds to the antigen, the T-line does not develop color, and only the quality control line (C-line) develops color to judge the result.

Compared with the traditional detection method, the pentazolyl alcohol colloidal gold rapid detection card has significant advantages. The first is the fast detection speed, usually within 10-15 minutes to produce results, without waiting for lengthy laboratory reaction time; Secondly, the operation is simple, without professional training and complex instruments, just simply extract the sample, add it dropwise to the test card, and observe the color development results with the naked eye; Furthermore, the cost is low, suitable for large-scale rapid screening; At the same time, it has good portability and can be used in field sites, market stalls and other on-site environments to meet the needs of rapid sampling.

In the application scenario, the pentazole colloidal gold rapid detection card is widely used in the field of agricultural product production bases for rapid testing, raw material inspection in the acquisition process, rapid screening by market supervision departments, and import and export inspection and quarantine. It can help producers keep abreast of product quality and avoid substandard products from entering the market; it can also provide efficient on-site testing support for regulatory authorities, quickly lock in risky products, and ensure consumers' "safety on the tip of the tongue".

Of course, the test results of the rapid test card can be used as a preliminary screening basis. If accurate quantification is required, it also needs to be combined with instrumental analysis methods such as high-performance liquid chromatography. However, under the demand of rapid, low-cost and on-site testing, the pentazole colloidal gold rapid test card is undoubtedly an indispensable part of the food safety prevention and control system, and plays an important role in safeguarding the safety of agricultural products.