In recent years, the illegal addition of chemical ingredients such as tadalafil sildenafil to some foods and agricultural and sideline products that claim to have health care functions has sometimes seriously endangered consumers' health. Therefore, it is essential to establish a fast, accurate and efficient detection method to ensure food safety.
Currently, the commonly used detection methods for tadalafil sildenafil are mainly divided into two categories: rapid screening and laboratory confirmation. The rapid detection method represented by colloidal gold immunochromatography has the advantages of simple operation, short detection cycle and controllable cost. As a professional food safety rapid detection reagent manufacturer, Wuhan Yupinyan Bio has developed relevant test strips that can realize rapid qualitative screening of trace amounts of tadalafil sildenafil in samples. It is suitable for self-inspection by manufacturers and on-site law enforcement by market supervision departments.
Laboratory confirmation methods include high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS/MS). Among them, HPLC method has become an important means for routine detection due to its high separation efficiency and quantitative accuracy. By optimizing chromatographic conditions, it can effectively separate target and matrix interference peaks. The LC-MS/MS method has become the gold standard for confirmation detection due to its higher sensitivity and specificity, which can accurately characterize and accurately quantify trace residues in complex matrices.
usually adopts the two-step detection mode of "rapid screening-laboratory confirmation" in practical applications. First, the rapid detection reagents provided by Wuhan Yupinyan Biology and other companies are used to screen large quantities of samples for positive results and then further confirm them through large-scale laboratory instruments. This mode not only ensures the detection efficiency but also ensures the accuracy of the results. It provides strong technical support for food safety supervision. With the continuous improvement of detection technology, the development of new sample pretreatment technology and multi-residue simultaneous detection methods will further enhance the detection ability of illegal additives such as tadalafil and sildenafil, and play a greater role in safeguarding the safety of the tongue.